Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32190

Experiment: 36hr Phyto Lib Only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32185 and BPHYT_RS32190 are separated by 1 nucleotidesBPHYT_RS32190 and BPHYT_RS32195 are separated by 16 nucleotidesBPHYT_RS32195 and BPHYT_RS32200 are separated by 41 nucleotides BPHYT_RS32185: BPHYT_RS32185 - type IV secretion protein VirB4, at 2,793,237 to 2,795,702 _RS32185 BPHYT_RS32190: BPHYT_RS32190 - conjugal transfer protein TraD, at 2,795,704 to 2,796,048 _RS32190 BPHYT_RS32195: BPHYT_RS32195 - type VI secretion protein, at 2,796,065 to 2,796,406 _RS32195 BPHYT_RS32200: BPHYT_RS32200 - lytic transglycosylase, at 2,796,448 to 2,797,092 _RS32200 Position (kb) 2795 2796 2797Strain fitness (log2 ratio) -1 0 1at 2794.705 kb on + strand, within BPHYT_RS32185at 2794.880 kb on - strand, within BPHYT_RS32185at 2794.895 kb on - strand, within BPHYT_RS32185at 2794.895 kb on - strand, within BPHYT_RS32185at 2794.895 kb on - strand, within BPHYT_RS32185at 2795.180 kb on - strand, within BPHYT_RS32185at 2795.223 kb on + strand, within BPHYT_RS32185at 2795.335 kb on + strand, within BPHYT_RS32185at 2795.336 kb on - strand, within BPHYT_RS32185at 2795.587 kb on + strandat 2795.798 kb on + strand, within BPHYT_RS32190at 2796.021 kb on + strandat 2796.025 kb on - strandat 2796.025 kb on - strandat 2796.601 kb on + strand, within BPHYT_RS32200at 2796.657 kb on - strand, within BPHYT_RS32200

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr Phyto Lib Only
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2,794,705 + BPHYT_RS32185 0.60 +1.0
2,794,880 - BPHYT_RS32185 0.67 -1.0
2,794,895 - BPHYT_RS32185 0.67 -0.4
2,794,895 - BPHYT_RS32185 0.67 +0.0
2,794,895 - BPHYT_RS32185 0.67 -0.4
2,795,180 - BPHYT_RS32185 0.79 +0.2
2,795,223 + BPHYT_RS32185 0.81 +0.2
2,795,335 + BPHYT_RS32185 0.85 +0.2
2,795,336 - BPHYT_RS32185 0.85 +0.5
2,795,587 + +0.5
2,795,798 + BPHYT_RS32190 0.27 +0.1
2,796,021 + -1.0
2,796,025 - +0.7
2,796,025 - +0.1
2,796,601 + BPHYT_RS32200 0.24 -0.7
2,796,657 - BPHYT_RS32200 0.32 +1.5

Or see this region's nucleotide sequence