Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10205

Experiment: 36hr Phyto Lib Only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10195 and BPHYT_RS10200 are separated by 490 nucleotidesBPHYT_RS10200 and BPHYT_RS10205 are separated by 43 nucleotidesBPHYT_RS10205 and BPHYT_RS10210 are separated by 151 nucleotides BPHYT_RS10195: BPHYT_RS10195 - major facilitator transporter, at 2,321,353 to 2,322,690 _RS10195 BPHYT_RS10200: BPHYT_RS10200 - short-chain dehydrogenase, at 2,323,181 to 2,323,561 _RS10200 BPHYT_RS10205: BPHYT_RS10205 - D-lactate dehydrogenase, at 2,323,605 to 2,325,344 _RS10205 BPHYT_RS10210: BPHYT_RS10210 - LysR family transcriptional regulator, at 2,325,496 to 2,326,395 _RS10210 Position (kb) 2323 2324 2325 2326Strain fitness (log2 ratio) -1 0 1at 2322.617 kb on + strandat 2322.857 kb on - strandat 2322.930 kb on - strandat 2322.954 kb on - strandat 2323.118 kb on + strandat 2323.119 kb on - strandat 2323.736 kb on + strandat 2324.398 kb on + strand, within BPHYT_RS10205at 2324.721 kb on - strand, within BPHYT_RS10205at 2324.814 kb on - strand, within BPHYT_RS10205at 2325.050 kb on - strand, within BPHYT_RS10205at 2325.050 kb on - strand, within BPHYT_RS10205at 2325.591 kb on - strand, within BPHYT_RS10210

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr Phyto Lib Only
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2,322,617 + -1.1
2,322,857 - +0.8
2,322,930 - -0.8
2,322,954 - -0.0
2,323,118 + -0.7
2,323,119 - -0.2
2,323,736 + +1.0
2,324,398 + BPHYT_RS10205 0.46 -0.1
2,324,721 - BPHYT_RS10205 0.64 -1.4
2,324,814 - BPHYT_RS10205 0.69 +0.1
2,325,050 - BPHYT_RS10205 0.83 +0.4
2,325,050 - BPHYT_RS10205 0.83 -0.7
2,325,591 - BPHYT_RS10210 0.11 -1.1

Or see this region's nucleotide sequence