Experiment: 36hr Phyto Lib Only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01855 and BPHYT_RS01860 are separated by 278 nucleotides BPHYT_RS01860 and BPHYT_RS01865 are separated by 235 nucleotides BPHYT_RS01865 and BPHYT_RS01870 are separated by 108 nucleotides
BPHYT_RS01855: BPHYT_RS01855 - FAD-dependent oxidoreductase, at 421,365 to 422,471
_RS01855
BPHYT_RS01860: BPHYT_RS01860 - high potential iron-sulfur protein, at 422,750 to 423,061
_RS01860
BPHYT_RS01865: BPHYT_RS01865 - major facilitator transporter, at 423,297 to 424,589
_RS01865
BPHYT_RS01870: BPHYT_RS01870 - 6-aminohexanoate-cyclic-dimer hydrolase, at 424,698 to 426,218
_RS01870
Position (kb) 423
424
425 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 422.315 kb on - strand, within BPHYT_RS01855 at 422.647 kb on - strand at 422.689 kb on - strand at 422.731 kb on - strand at 422.731 kb on - strand at 422.732 kb on + strand at 422.880 kb on - strand, within BPHYT_RS01860 at 422.924 kb on + strand, within BPHYT_RS01860 at 422.925 kb on - strand, within BPHYT_RS01860 at 422.953 kb on + strand, within BPHYT_RS01860 at 422.954 kb on - strand, within BPHYT_RS01860 at 422.954 kb on - strand, within BPHYT_RS01860 at 422.960 kb on + strand, within BPHYT_RS01860 at 423.044 kb on + strand at 423.044 kb on + strand at 423.045 kb on - strand at 423.047 kb on - strand at 423.206 kb on - strand at 423.253 kb on - strand at 423.265 kb on + strand at 423.266 kb on - strand at 423.266 kb on - strand at 423.272 kb on + strand at 423.370 kb on - strand at 423.509 kb on - strand, within BPHYT_RS01865 at 423.519 kb on + strand, within BPHYT_RS01865 at 423.520 kb on - strand, within BPHYT_RS01865 at 423.647 kb on - strand, within BPHYT_RS01865 at 423.900 kb on + strand, within BPHYT_RS01865 at 423.901 kb on - strand, within BPHYT_RS01865 at 424.047 kb on + strand, within BPHYT_RS01865 at 424.047 kb on + strand, within BPHYT_RS01865 at 424.048 kb on - strand, within BPHYT_RS01865 at 424.063 kb on - strand, within BPHYT_RS01865 at 424.063 kb on - strand, within BPHYT_RS01865 at 424.101 kb on + strand, within BPHYT_RS01865 at 424.223 kb on + strand, within BPHYT_RS01865 at 424.224 kb on - strand, within BPHYT_RS01865 at 424.239 kb on + strand, within BPHYT_RS01865 at 424.240 kb on - strand, within BPHYT_RS01865 at 424.314 kb on - strand, within BPHYT_RS01865 at 424.327 kb on - strand, within BPHYT_RS01865 at 424.329 kb on + strand, within BPHYT_RS01865 at 424.401 kb on + strand, within BPHYT_RS01865 at 424.402 kb on - strand, within BPHYT_RS01865 at 424.491 kb on + strand at 424.492 kb on - strand at 424.492 kb on - strand at 424.495 kb on - strand at 424.699 kb on + strand at 424.700 kb on - strand at 424.700 kb on - strand at 424.844 kb on - strand at 425.321 kb on + strand, within BPHYT_RS01870 at 425.321 kb on + strand, within BPHYT_RS01870 at 425.322 kb on - strand, within BPHYT_RS01870 at 425.322 kb on - strand, within BPHYT_RS01870 at 425.413 kb on + strand, within BPHYT_RS01870 at 425.420 kb on + strand, within BPHYT_RS01870
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr Phyto Lib Only remove 422,315 - BPHYT_RS01855 0.86 -0.5 422,647 - -1.3 422,689 - -0.5 422,731 - +0.2 422,731 - -0.2 422,732 + -0.5 422,880 - BPHYT_RS01860 0.42 -0.6 422,924 + BPHYT_RS01860 0.56 -0.9 422,925 - BPHYT_RS01860 0.56 -0.0 422,953 + BPHYT_RS01860 0.65 +0.3 422,954 - BPHYT_RS01860 0.65 +0.4 422,954 - BPHYT_RS01860 0.65 -0.6 422,960 + BPHYT_RS01860 0.67 -0.4 423,044 + +0.1 423,044 + -0.3 423,045 - -1.9 423,047 - -0.5 423,206 - -0.2 423,253 - +0.4 423,265 + +0.9 423,266 - -0.1 423,266 - -1.4 423,272 + -0.7 423,370 - +0.4 423,509 - BPHYT_RS01865 0.16 +1.0 423,519 + BPHYT_RS01865 0.17 -1.8 423,520 - BPHYT_RS01865 0.17 -1.8 423,647 - BPHYT_RS01865 0.27 +0.6 423,900 + BPHYT_RS01865 0.47 +0.8 423,901 - BPHYT_RS01865 0.47 -0.4 424,047 + BPHYT_RS01865 0.58 -1.4 424,047 + BPHYT_RS01865 0.58 -0.1 424,048 - BPHYT_RS01865 0.58 +0.9 424,063 - BPHYT_RS01865 0.59 +3.3 424,063 - BPHYT_RS01865 0.59 +0.5 424,101 + BPHYT_RS01865 0.62 +0.1 424,223 + BPHYT_RS01865 0.72 -0.1 424,224 - BPHYT_RS01865 0.72 -0.0 424,239 + BPHYT_RS01865 0.73 -1.0 424,240 - BPHYT_RS01865 0.73 +0.1 424,314 - BPHYT_RS01865 0.79 +0.5 424,327 - BPHYT_RS01865 0.80 -0.4 424,329 + BPHYT_RS01865 0.80 -1.6 424,401 + BPHYT_RS01865 0.85 +0.6 424,402 - BPHYT_RS01865 0.85 +0.1 424,491 + -0.9 424,492 - +0.2 424,492 - +0.9 424,495 - -0.5 424,699 + +0.3 424,700 - -0.4 424,700 - -0.4 424,844 - +0.4 425,321 + BPHYT_RS01870 0.41 -3.3 425,321 + BPHYT_RS01870 0.41 -0.4 425,322 - BPHYT_RS01870 0.41 -0.7 425,322 - BPHYT_RS01870 0.41 +0.3 425,413 + BPHYT_RS01870 0.47 -0.1 425,420 + BPHYT_RS01870 0.47 +0.7
Or see this region's nucleotide sequence