Experiment: 36hr Phyto Lib Only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01250 and BPHYT_RS01255 are separated by 285 nucleotides BPHYT_RS01255 and BPHYT_RS01260 are separated by 215 nucleotides BPHYT_RS01260 and BPHYT_RS01265 are separated by 38 nucleotides
BPHYT_RS01250: BPHYT_RS01250 - integrase, at 286,317 to 287,315
_RS01250
BPHYT_RS01255: BPHYT_RS01255 - AsnC family transcriptional regulator, at 287,601 to 288,062
_RS01255
BPHYT_RS01260: BPHYT_RS01260 - hypothetical protein, at 288,278 to 289,408
_RS01260
BPHYT_RS01265: BPHYT_RS01265 - lysine transporter LysE, at 289,447 to 290,061
_RS01265
Position (kb) 288
289
290 Strain fitness (log2 ratio) -2
-1
0
1
2 at 287.533 kb on - strand at 287.652 kb on - strand, within BPHYT_RS01255 at 287.717 kb on - strand, within BPHYT_RS01255 at 287.717 kb on - strand, within BPHYT_RS01255 at 287.756 kb on - strand, within BPHYT_RS01255 at 287.852 kb on - strand, within BPHYT_RS01255 at 287.973 kb on + strand, within BPHYT_RS01255 at 288.074 kb on + strand at 288.075 kb on - strand at 288.142 kb on - strand at 288.142 kb on - strand at 288.175 kb on + strand at 288.301 kb on + strand at 288.301 kb on + strand at 288.301 kb on + strand at 288.302 kb on - strand at 288.360 kb on + strand at 288.398 kb on + strand, within BPHYT_RS01260 at 288.446 kb on + strand, within BPHYT_RS01260 at 288.569 kb on + strand, within BPHYT_RS01260 at 288.724 kb on - strand, within BPHYT_RS01260 at 288.725 kb on + strand, within BPHYT_RS01260 at 288.986 kb on + strand, within BPHYT_RS01260 at 288.987 kb on - strand, within BPHYT_RS01260 at 289.058 kb on + strand, within BPHYT_RS01260 at 289.059 kb on - strand, within BPHYT_RS01260 at 289.074 kb on - strand, within BPHYT_RS01260 at 289.075 kb on + strand, within BPHYT_RS01260 at 289.075 kb on + strand, within BPHYT_RS01260 at 289.159 kb on + strand, within BPHYT_RS01260 at 289.159 kb on + strand, within BPHYT_RS01260 at 289.159 kb on + strand, within BPHYT_RS01260 at 289.159 kb on + strand, within BPHYT_RS01260 at 289.160 kb on - strand, within BPHYT_RS01260 at 289.373 kb on + strand at 289.374 kb on - strand at 289.418 kb on + strand at 289.434 kb on - strand at 289.435 kb on + strand at 289.436 kb on - strand at 289.446 kb on + strand at 289.446 kb on + strand at 289.446 kb on + strand at 289.447 kb on - strand at 289.447 kb on - strand at 289.571 kb on + strand, within BPHYT_RS01265 at 289.704 kb on - strand, within BPHYT_RS01265 at 289.708 kb on + strand, within BPHYT_RS01265 at 289.709 kb on - strand, within BPHYT_RS01265 at 289.810 kb on - strand, within BPHYT_RS01265 at 290.081 kb on + strand at 290.150 kb on + strand at 290.217 kb on + strand at 290.217 kb on + strand at 290.255 kb on + strand at 290.348 kb on - strand at 290.360 kb on - strand at 290.360 kb on - strand at 290.360 kb on - strand at 290.388 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr Phyto Lib Only remove 287,533 - -0.3 287,652 - BPHYT_RS01255 0.11 -0.3 287,717 - BPHYT_RS01255 0.25 -1.5 287,717 - BPHYT_RS01255 0.25 -0.3 287,756 - BPHYT_RS01255 0.34 -0.3 287,852 - BPHYT_RS01255 0.54 -0.6 287,973 + BPHYT_RS01255 0.81 -0.1 288,074 + +0.1 288,075 - +0.5 288,142 - +0.6 288,142 - +0.4 288,175 + -0.1 288,301 + +0.7 288,301 + -0.3 288,301 + +0.6 288,302 - +2.0 288,360 + -0.9 288,398 + BPHYT_RS01260 0.11 +0.2 288,446 + BPHYT_RS01260 0.15 +0.5 288,569 + BPHYT_RS01260 0.26 +0.1 288,724 - BPHYT_RS01260 0.39 +0.1 288,725 + BPHYT_RS01260 0.40 -1.9 288,986 + BPHYT_RS01260 0.63 +0.5 288,987 - BPHYT_RS01260 0.63 -0.0 289,058 + BPHYT_RS01260 0.69 +1.5 289,059 - BPHYT_RS01260 0.69 +0.8 289,074 - BPHYT_RS01260 0.70 -1.2 289,075 + BPHYT_RS01260 0.70 +0.7 289,075 + BPHYT_RS01260 0.70 -0.3 289,159 + BPHYT_RS01260 0.78 +0.2 289,159 + BPHYT_RS01260 0.78 +1.5 289,159 + BPHYT_RS01260 0.78 -0.2 289,159 + BPHYT_RS01260 0.78 -0.0 289,160 - BPHYT_RS01260 0.78 +0.6 289,373 + +0.0 289,374 - -0.5 289,418 + -0.1 289,434 - -0.0 289,435 + -0.2 289,436 - +0.8 289,446 + -0.6 289,446 + -0.9 289,446 + +0.1 289,447 - -1.0 289,447 - +0.2 289,571 + BPHYT_RS01265 0.20 -0.4 289,704 - BPHYT_RS01265 0.42 -0.8 289,708 + BPHYT_RS01265 0.42 +0.4 289,709 - BPHYT_RS01265 0.43 -0.5 289,810 - BPHYT_RS01265 0.59 -0.0 290,081 + +0.8 290,150 + -0.1 290,217 + -0.5 290,217 + +0.4 290,255 + -0.9 290,348 - -0.0 290,360 - -0.5 290,360 - -2.7 290,360 - +0.8 290,388 - -0.2
Or see this region's nucleotide sequence