Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32335

Experiment: 36hr PHYTO6 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32330 and BPHYT_RS32335 are separated by 333 nucleotidesBPHYT_RS32335 and BPHYT_RS32340 are separated by 133 nucleotidesBPHYT_RS32340 and BPHYT_RS32345 overlap by 4 nucleotides BPHYT_RS32330: BPHYT_RS32330 - 5-oxoprolinase, at 2,825,086 to 2,827,122 _RS32330 BPHYT_RS32335: BPHYT_RS32335 - AsnC family transcriptional regulator, at 2,827,456 to 2,827,881 _RS32335 BPHYT_RS32340: BPHYT_RS32340 - LrgA family protein, at 2,828,015 to 2,828,419 _RS32340 BPHYT_RS32345: BPHYT_RS32345 - LrgB family protein, at 2,828,416 to 2,829,096 _RS32345 Position (kb) 2827 2828Strain fitness (log2 ratio) -1 0 1at 2826.467 kb on + strand, within BPHYT_RS32330at 2826.517 kb on - strand, within BPHYT_RS32330at 2826.524 kb on + strand, within BPHYT_RS32330at 2826.618 kb on + strand, within BPHYT_RS32330at 2826.844 kb on + strand, within BPHYT_RS32330at 2826.845 kb on - strand, within BPHYT_RS32330at 2827.105 kb on + strandat 2827.544 kb on - strand, within BPHYT_RS32335at 2827.580 kb on - strand, within BPHYT_RS32335at 2828.037 kb on + strandat 2828.109 kb on + strand, within BPHYT_RS32340at 2828.110 kb on - strand, within BPHYT_RS32340at 2828.237 kb on - strand, within BPHYT_RS32340at 2828.351 kb on - strand, within BPHYT_RS32340at 2828.479 kb on + strandat 2828.480 kb on - strandat 2828.480 kb on - strandat 2828.567 kb on - strand, within BPHYT_RS32345at 2828.576 kb on - strand, within BPHYT_RS32345at 2828.576 kb on - strand, within BPHYT_RS32345at 2828.661 kb on + strand, within BPHYT_RS32345

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 0.1
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2,826,467 + BPHYT_RS32330 0.68 -1.4
2,826,517 - BPHYT_RS32330 0.70 -0.4
2,826,524 + BPHYT_RS32330 0.71 -0.5
2,826,618 + BPHYT_RS32330 0.75 -0.4
2,826,844 + BPHYT_RS32330 0.86 -0.6
2,826,845 - BPHYT_RS32330 0.86 +0.5
2,827,105 + -0.1
2,827,544 - BPHYT_RS32335 0.21 +0.7
2,827,580 - BPHYT_RS32335 0.29 +0.6
2,828,037 + -0.4
2,828,109 + BPHYT_RS32340 0.23 -0.6
2,828,110 - BPHYT_RS32340 0.23 +1.4
2,828,237 - BPHYT_RS32340 0.55 +0.0
2,828,351 - BPHYT_RS32340 0.83 -1.0
2,828,479 + -0.7
2,828,480 - -0.2
2,828,480 - -0.3
2,828,567 - BPHYT_RS32345 0.22 +0.0
2,828,576 - BPHYT_RS32345 0.23 +0.1
2,828,576 - BPHYT_RS32345 0.23 -0.1
2,828,661 + BPHYT_RS32345 0.36 -0.3

Or see this region's nucleotide sequence