Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS30465

Experiment: 36hr PHYTO6 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS30460 and BPHYT_RS30465 are separated by 58 nucleotidesBPHYT_RS30465 and BPHYT_RS30470 are separated by 197 nucleotidesBPHYT_RS30470 and BPHYT_RS30475 are separated by 86 nucleotides BPHYT_RS30460: BPHYT_RS30460 - glycerate dehydrogenase, at 2,393,594 to 2,394,559 _RS30460 BPHYT_RS30465: BPHYT_RS30465 - short-chain dehydrogenase, at 2,394,618 to 2,395,394 _RS30465 BPHYT_RS30470: BPHYT_RS30470 - TetR family transcriptional regulator, at 2,395,592 to 2,396,179 _RS30470 BPHYT_RS30475: BPHYT_RS30475 - pyruvate-binding protein, at 2,396,266 to 2,397,750 _RS30475 Position (kb) 2394 2395 2396Strain fitness (log2 ratio) -1 0 1 2at 2394.232 kb on + strand, within BPHYT_RS30460at 2394.950 kb on - strand, within BPHYT_RS30465at 2395.598 kb on + strandat 2396.375 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 0.1
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2,394,232 + BPHYT_RS30460 0.66 -0.6
2,394,950 - BPHYT_RS30465 0.43 +0.7
2,395,598 + +2.0
2,396,375 + -0.6

Or see this region's nucleotide sequence