Experiment: 36hr PHYTO6 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28285 and BPHYT_RS28290 are separated by 382 nucleotides BPHYT_RS28290 and BPHYT_RS28295 are separated by 59 nucleotides BPHYT_RS28295 and BPHYT_RS28300 are separated by 811 nucleotides
BPHYT_RS28285: BPHYT_RS28285 - MFS transporter, at 1,901,509 to 1,902,756
_RS28285
BPHYT_RS28290: BPHYT_RS28290 - hypothetical protein, at 1,903,139 to 1,903,411
_RS28290
BPHYT_RS28295: BPHYT_RS28295 - hypothetical protein, at 1,903,471 to 1,903,788
_RS28295
BPHYT_RS28300: BPHYT_RS28300 - histone nucleoid-structuring protein H-NS, at 1,904,600 to 1,904,902
_RS28300
Position (kb)
1903
1904 Strain fitness (log2 ratio)
-1
0
1 at 1902.756 kb on + strand at 1902.756 kb on + strand at 1902.757 kb on - strand at 1902.757 kb on - strand at 1902.759 kb on + strand at 1902.774 kb on + strand at 1902.774 kb on + strand at 1902.778 kb on - strand at 1902.778 kb on - strand at 1902.932 kb on + strand at 1902.932 kb on + strand at 1902.991 kb on - strand at 1903.145 kb on - strand at 1903.145 kb on - strand at 1903.205 kb on + strand, within BPHYT_RS28290 at 1903.206 kb on - strand, within BPHYT_RS28290 at 1903.484 kb on + strand at 1903.774 kb on + strand at 1903.774 kb on + strand at 1903.781 kb on - strand at 1903.781 kb on - strand at 1903.921 kb on + strand at 1904.034 kb on + strand at 1904.035 kb on - strand at 1904.102 kb on + strand at 1904.175 kb on - strand at 1904.205 kb on - strand at 1904.420 kb on + strand at 1904.494 kb on + strand at 1904.535 kb on - strand at 1904.722 kb on + strand, within BPHYT_RS28300
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 0.1 remove 1,902,756 + +0.5 1,902,756 + -1.3 1,902,757 - -0.3 1,902,757 - +0.0 1,902,759 + -0.5 1,902,774 + -0.9 1,902,774 + +0.0 1,902,778 - +0.2 1,902,778 - +0.2 1,902,932 + -0.8 1,902,932 + -0.4 1,902,991 - +0.6 1,903,145 - +0.7 1,903,145 - -0.2 1,903,205 + BPHYT_RS28290 0.24 +0.0 1,903,206 - BPHYT_RS28290 0.25 +0.4 1,903,484 + +0.6 1,903,774 + +1.1 1,903,774 + -0.2 1,903,781 - -0.2 1,903,781 - -0.4 1,903,921 + +0.7 1,904,034 + +1.2 1,904,035 - -0.0 1,904,102 + -1.7 1,904,175 - -0.5 1,904,205 - +0.1 1,904,420 + -0.3 1,904,494 + +0.9 1,904,535 - +0.8 1,904,722 + BPHYT_RS28300 0.40 -0.5
Or see this region's nucleotide sequence