Experiment: 36hr PHYTO6 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22965 and BPHYT_RS22970 are separated by 82 nucleotides BPHYT_RS22970 and BPHYT_RS22975 are separated by 273 nucleotides BPHYT_RS22975 and BPHYT_RS22980 are separated by 52 nucleotides
BPHYT_RS22965: BPHYT_RS22965 - ligand-gated channel protein, at 710,220 to 712,634
_RS22965
BPHYT_RS22970: BPHYT_RS22970 - hypothetical protein, at 712,717 to 713,127
_RS22970
BPHYT_RS22975: BPHYT_RS22975 - hypothetical protein, at 713,401 to 715,545
_RS22975
BPHYT_RS22980: BPHYT_RS22980 - LysR family transcriptional regulator, at 715,598 to 716,494
_RS22980
Position (kb) 713
714
715
716 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 712.538 kb on - strand at 713.139 kb on + strand at 713.140 kb on - strand at 713.157 kb on + strand at 713.195 kb on + strand at 713.272 kb on + strand at 713.272 kb on + strand at 713.273 kb on - strand at 713.713 kb on + strand, within BPHYT_RS22975 at 713.815 kb on + strand, within BPHYT_RS22975 at 713.846 kb on - strand, within BPHYT_RS22975 at 713.860 kb on + strand, within BPHYT_RS22975 at 714.268 kb on + strand, within BPHYT_RS22975 at 714.269 kb on - strand, within BPHYT_RS22975 at 714.472 kb on + strand, within BPHYT_RS22975 at 714.472 kb on + strand, within BPHYT_RS22975 at 714.479 kb on + strand, within BPHYT_RS22975 at 714.549 kb on - strand, within BPHYT_RS22975 at 715.137 kb on - strand, within BPHYT_RS22975 at 715.324 kb on - strand, within BPHYT_RS22975 at 715.534 kb on - strand at 715.539 kb on + strand at 715.674 kb on + strand at 716.529 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 0.1 remove 712,538 - +0.7 713,139 + +0.2 713,140 - -1.1 713,157 + +0.2 713,195 + -0.3 713,272 + -0.6 713,272 + -0.6 713,273 - -1.0 713,713 + BPHYT_RS22975 0.15 -0.6 713,815 + BPHYT_RS22975 0.19 -0.9 713,846 - BPHYT_RS22975 0.21 +0.2 713,860 + BPHYT_RS22975 0.21 +0.3 714,268 + BPHYT_RS22975 0.40 -0.7 714,269 - BPHYT_RS22975 0.40 -0.6 714,472 + BPHYT_RS22975 0.50 +4.0 714,472 + BPHYT_RS22975 0.50 -0.1 714,479 + BPHYT_RS22975 0.50 +0.7 714,549 - BPHYT_RS22975 0.54 -0.5 715,137 - BPHYT_RS22975 0.81 -1.2 715,324 - BPHYT_RS22975 0.90 +0.1 715,534 - +1.9 715,539 + +0.6 715,674 + +0.0 716,529 + -0.1
Or see this region's nucleotide sequence