Experiment: 36hr PHYTO6 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22095 and BPHYT_RS22100 are separated by 418 nucleotides BPHYT_RS22100 and BPHYT_RS22105 are separated by 273 nucleotides
BPHYT_RS22095: BPHYT_RS22095 - porin, at 514,363 to 515,544
_RS22095
BPHYT_RS22100: BPHYT_RS22100 - ribonuclease BN, at 515,963 to 517,294
_RS22100
BPHYT_RS22105: BPHYT_RS22105 - tRNA synthetase, at 517,568 to 518,107
_RS22105
Position (kb) 515
516
517
518 Strain fitness (log2 ratio) -2
-1
0
1
2 at 515.012 kb on + strand, within BPHYT_RS22095 at 515.012 kb on + strand at 515.013 kb on - strand, within BPHYT_RS22095 at 515.013 kb on - strand, within BPHYT_RS22095 at 515.013 kb on - strand, within BPHYT_RS22095 at 515.054 kb on + strand, within BPHYT_RS22095 at 515.055 kb on - strand, within BPHYT_RS22095 at 515.200 kb on - strand, within BPHYT_RS22095 at 515.432 kb on + strand at 515.433 kb on - strand at 515.459 kb on + strand at 515.523 kb on + strand at 515.567 kb on - strand at 515.592 kb on + strand at 515.592 kb on + strand at 515.760 kb on + strand at 515.760 kb on + strand at 515.760 kb on + strand at 515.761 kb on - strand at 515.796 kb on + strand at 515.797 kb on - strand at 515.805 kb on + strand at 515.805 kb on + strand at 515.805 kb on + strand at 516.064 kb on - strand at 516.489 kb on + strand, within BPHYT_RS22100 at 516.489 kb on + strand, within BPHYT_RS22100 at 516.562 kb on - strand, within BPHYT_RS22100 at 516.608 kb on + strand, within BPHYT_RS22100 at 516.736 kb on - strand, within BPHYT_RS22100 at 516.916 kb on + strand, within BPHYT_RS22100 at 517.164 kb on + strand at 517.165 kb on - strand at 517.461 kb on - strand at 517.461 kb on - strand at 517.461 kb on - strand at 517.461 kb on - strand at 517.462 kb on + strand at 517.463 kb on - strand at 517.463 kb on - strand at 517.532 kb on + strand at 517.533 kb on - strand at 517.553 kb on + strand at 517.554 kb on - strand at 517.554 kb on - strand at 517.568 kb on - strand at 517.587 kb on + strand at 517.588 kb on - strand at 517.772 kb on + strand, within BPHYT_RS22105 at 518.052 kb on + strand, within BPHYT_RS22105 at 518.053 kb on - strand, within BPHYT_RS22105 at 518.053 kb on - strand, within BPHYT_RS22105 at 518.105 kb on + strand at 518.209 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 0.1 remove 515,012 + BPHYT_RS22095 0.55 +0.3 515,012 + +0.8 515,013 - BPHYT_RS22095 0.55 -0.0 515,013 - BPHYT_RS22095 0.55 -1.8 515,013 - BPHYT_RS22095 0.55 +1.0 515,054 + BPHYT_RS22095 0.58 +0.8 515,055 - BPHYT_RS22095 0.59 -1.4 515,200 - BPHYT_RS22095 0.71 +0.5 515,432 + -1.0 515,433 - -0.7 515,459 + +0.5 515,523 + +1.0 515,567 - -1.5 515,592 + -0.4 515,592 + -1.2 515,760 + +0.2 515,760 + -0.5 515,760 + +1.5 515,761 - +0.7 515,796 + +0.1 515,797 - -0.6 515,805 + -0.4 515,805 + -0.5 515,805 + +0.4 516,064 - -0.1 516,489 + BPHYT_RS22100 0.39 +0.0 516,489 + BPHYT_RS22100 0.39 +0.2 516,562 - BPHYT_RS22100 0.45 +1.4 516,608 + BPHYT_RS22100 0.48 -0.4 516,736 - BPHYT_RS22100 0.58 +0.8 516,916 + BPHYT_RS22100 0.72 +1.9 517,164 + -0.1 517,165 - -0.6 517,461 - +0.2 517,461 - -1.0 517,461 - +1.4 517,461 - -2.0 517,462 + -0.9 517,463 - +1.1 517,463 - -0.5 517,532 + +0.3 517,533 - +0.0 517,553 + +0.0 517,554 - +1.5 517,554 - +0.5 517,568 - -0.2 517,587 + +0.2 517,588 - +0.0 517,772 + BPHYT_RS22105 0.38 -0.2 518,052 + BPHYT_RS22105 0.90 -1.1 518,053 - BPHYT_RS22105 0.90 +0.0 518,053 - BPHYT_RS22105 0.90 +0.3 518,105 + -0.2 518,209 - +2.0
Or see this region's nucleotide sequence