Experiment: 36hr PHYTO6 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21190 and BPHYT_RS21195 are separated by 495 nucleotides BPHYT_RS21195 and BPHYT_RS21200 are separated by 123 nucleotides BPHYT_RS21200 and BPHYT_RS21205 overlap by 7 nucleotides
BPHYT_RS21190: BPHYT_RS21190 - phospholipase C, at 315,315 to 317,000
_RS21190
BPHYT_RS21195: BPHYT_RS21195 - hypothetical protein, at 317,496 to 318,581
_RS21195
BPHYT_RS21200: BPHYT_RS21200 - hypothetical protein, at 318,705 to 319,337
_RS21200
BPHYT_RS21205: BPHYT_RS21205 - high-affinity nickel-transporter, at 319,331 to 320,455
_RS21205
Position (kb) 317
318
319 Strain fitness (log2 ratio) -1
0
1 at 316.496 kb on - strand, within BPHYT_RS21190 at 316.498 kb on + strand, within BPHYT_RS21190 at 316.594 kb on + strand, within BPHYT_RS21190 at 317.987 kb on - strand, within BPHYT_RS21195 at 318.248 kb on + strand, within BPHYT_RS21195 at 318.390 kb on + strand, within BPHYT_RS21195 at 318.484 kb on - strand at 318.584 kb on - strand at 319.014 kb on + strand, within BPHYT_RS21200 at 319.106 kb on + strand, within BPHYT_RS21200 at 319.229 kb on - strand, within BPHYT_RS21200 at 319.304 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 0.1 remove 316,496 - BPHYT_RS21190 0.70 -0.5 316,498 + BPHYT_RS21190 0.70 +1.2 316,594 + BPHYT_RS21190 0.76 +1.4 317,987 - BPHYT_RS21195 0.45 -0.9 318,248 + BPHYT_RS21195 0.69 -0.6 318,390 + BPHYT_RS21195 0.82 -0.0 318,484 - -1.4 318,584 - +0.5 319,014 + BPHYT_RS21200 0.49 +0.4 319,106 + BPHYT_RS21200 0.63 -0.6 319,229 - BPHYT_RS21200 0.83 +0.3 319,304 + +0.3
Or see this region's nucleotide sequence