Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS05325

Experiment: 36hr PHYTO6 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS05315 and BPHYT_RS05320 are separated by 48 nucleotidesBPHYT_RS05320 and BPHYT_RS05325 are separated by 87 nucleotidesBPHYT_RS05325 and BPHYT_RS05330 are separated by 861 nucleotides BPHYT_RS05315: BPHYT_RS05315 - hydrolase, at 1,224,016 to 1,225,179 _RS05315 BPHYT_RS05320: BPHYT_RS05320 - beta-lactamase, at 1,225,228 to 1,226,019 _RS05320 BPHYT_RS05325: BPHYT_RS05325 - amidohydrolase, at 1,226,107 to 1,226,526 _RS05325 BPHYT_RS05330: BPHYT_RS05330 - phosphohydrolase, at 1,227,388 to 1,228,029 _RS05330 Position (kb) 1226 1227Strain fitness (log2 ratio) -1 0 1at 1225.234 kb on - strandat 1225.234 kb on - strandat 1225.248 kb on + strandat 1225.249 kb on - strandat 1225.528 kb on + strand, within BPHYT_RS05320at 1225.528 kb on + strand, within BPHYT_RS05320at 1226.014 kb on - strandat 1226.281 kb on + strand, within BPHYT_RS05325at 1226.281 kb on + strand, within BPHYT_RS05325at 1226.342 kb on - strand, within BPHYT_RS05325at 1226.439 kb on + strand, within BPHYT_RS05325at 1226.460 kb on - strand, within BPHYT_RS05325at 1226.510 kb on - strandat 1226.538 kb on + strandat 1226.814 kb on - strandat 1226.829 kb on + strandat 1226.830 kb on - strandat 1226.831 kb on + strandat 1226.831 kb on + strandat 1226.831 kb on + strandat 1226.895 kb on - strandat 1226.994 kb on - strandat 1227.014 kb on + strandat 1227.034 kb on - strandat 1227.284 kb on - strandat 1227.314 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 0.1
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1,225,234 - +0.3
1,225,234 - +0.2
1,225,248 + -1.0
1,225,249 - -1.3
1,225,528 + BPHYT_RS05320 0.38 -0.0
1,225,528 + BPHYT_RS05320 0.38 +1.3
1,226,014 - -1.0
1,226,281 + BPHYT_RS05325 0.41 -0.5
1,226,281 + BPHYT_RS05325 0.41 -0.7
1,226,342 - BPHYT_RS05325 0.56 -0.2
1,226,439 + BPHYT_RS05325 0.79 +1.3
1,226,460 - BPHYT_RS05325 0.84 +0.7
1,226,510 - -0.1
1,226,538 + -0.0
1,226,814 - -0.4
1,226,829 + -0.3
1,226,830 - -0.3
1,226,831 + -0.5
1,226,831 + -0.1
1,226,831 + -0.1
1,226,895 - +0.3
1,226,994 - -1.3
1,227,014 + -0.2
1,227,034 - -0.6
1,227,284 - -0.7
1,227,314 + +0.0

Or see this region's nucleotide sequence