Experiment: 36hr PHYTO6 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03160 and BPHYT_RS03165 are separated by 67 nucleotides BPHYT_RS03165 and BPHYT_RS03170 are separated by 38 nucleotides BPHYT_RS03170 and BPHYT_RS03175 are separated by 157 nucleotides BPHYT_RS03175 and BPHYT_RS03180 are separated by 15 nucleotides
BPHYT_RS03160: BPHYT_RS03160 - hypothetical protein, at 709,627 to 710,040
_RS03160
BPHYT_RS03165: BPHYT_RS03165 - ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE, at 710,108 to 710,839
_RS03165
BPHYT_RS03170: BPHYT_RS03170 - membrane protein, at 710,878 to 711,870
_RS03170
BPHYT_RS03175: BPHYT_RS03175 - sterol-binding protein, at 712,028 to 712,672
_RS03175
BPHYT_RS03180: BPHYT_RS03180 - hypothetical protein, at 712,688 to 714,265
_RS03180
Position (kb) 710
711
712 Strain fitness (log2 ratio) -1
0
1
2
3 at 709.900 kb on + strand, within BPHYT_RS03160 at 709.900 kb on + strand, within BPHYT_RS03160 at 709.900 kb on + strand, within BPHYT_RS03160 at 710.041 kb on + strand at 710.041 kb on + strand at 710.088 kb on + strand at 710.835 kb on - strand at 711.042 kb on + strand, within BPHYT_RS03170 at 711.316 kb on - strand, within BPHYT_RS03170 at 711.539 kb on - strand, within BPHYT_RS03170 at 711.945 kb on + strand at 711.971 kb on + strand at 711.971 kb on + strand at 711.971 kb on + strand at 711.971 kb on + strand at 711.972 kb on - strand at 712.263 kb on + strand, within BPHYT_RS03175 at 712.263 kb on + strand, within BPHYT_RS03175 at 712.670 kb on + strand at 712.670 kb on + strand at 712.687 kb on + strand at 712.687 kb on + strand at 712.687 kb on + strand at 712.687 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 0.1 remove 709,900 + BPHYT_RS03160 0.66 +0.4 709,900 + BPHYT_RS03160 0.66 +0.3 709,900 + BPHYT_RS03160 0.66 -0.4 710,041 + -1.2 710,041 + -0.4 710,088 + -0.2 710,835 - +1.3 711,042 + BPHYT_RS03170 0.17 -1.3 711,316 - BPHYT_RS03170 0.44 +2.3 711,539 - BPHYT_RS03170 0.67 +1.9 711,945 + +2.0 711,971 + -0.4 711,971 + +0.2 711,971 + +1.0 711,971 + +0.2 711,972 - +0.1 712,263 + BPHYT_RS03175 0.36 +0.2 712,263 + BPHYT_RS03175 0.36 -0.3 712,670 + +3.0 712,670 + -0.3 712,687 + -0.5 712,687 + +0.0 712,687 + +1.0 712,687 + +1.3
Or see this region's nucleotide sequence