Experiment: 36hr PHYTO6 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01290 and BPHYT_RS01295 are separated by 61 nucleotides BPHYT_RS01295 and BPHYT_RS01300 are separated by 87 nucleotides BPHYT_RS01300 and BPHYT_RS01305 are separated by 116 nucleotides
BPHYT_RS01290: BPHYT_RS01290 - FAD dependent oxidoreductase, at 294,921 to 296,360
_RS01290
BPHYT_RS01295: BPHYT_RS01295 - glutathione S-transferase, at 296,422 to 297,096
_RS01295
BPHYT_RS01300: BPHYT_RS01300 - ArsR family transcriptional regulator, at 297,184 to 297,879
_RS01300
BPHYT_RS01305: BPHYT_RS01305 - MFS transporter, at 297,996 to 299,387
_RS01305
Position (kb) 297
298 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 296.278 kb on - strand at 296.292 kb on - strand at 296.307 kb on + strand at 296.308 kb on - strand at 296.335 kb on + strand at 296.437 kb on + strand at 296.460 kb on + strand at 296.460 kb on + strand at 296.461 kb on - strand at 296.480 kb on + strand at 296.639 kb on - strand, within BPHYT_RS01295 at 296.747 kb on + strand, within BPHYT_RS01295 at 296.747 kb on + strand, within BPHYT_RS01295 at 296.748 kb on - strand, within BPHYT_RS01295 at 296.819 kb on + strand, within BPHYT_RS01295 at 296.826 kb on + strand, within BPHYT_RS01295 at 297.059 kb on + strand at 297.094 kb on + strand at 297.193 kb on - strand at 297.196 kb on - strand at 297.339 kb on - strand, within BPHYT_RS01300 at 297.542 kb on + strand, within BPHYT_RS01300 at 297.672 kb on - strand, within BPHYT_RS01300 at 297.672 kb on - strand, within BPHYT_RS01300 at 297.811 kb on - strand at 297.864 kb on + strand at 297.864 kb on + strand at 297.864 kb on + strand at 297.865 kb on - strand at 297.897 kb on + strand at 297.907 kb on + strand at 297.976 kb on - strand at 297.993 kb on + strand at 298.045 kb on + strand at 298.082 kb on - strand at 298.085 kb on + strand at 298.085 kb on + strand at 298.085 kb on + strand at 298.086 kb on - strand at 298.086 kb on - strand at 298.139 kb on + strand, within BPHYT_RS01305 at 298.171 kb on - strand, within BPHYT_RS01305 at 298.173 kb on - strand, within BPHYT_RS01305 at 298.173 kb on - strand, within BPHYT_RS01305 at 298.382 kb on + strand, within BPHYT_RS01305 at 298.382 kb on + strand, within BPHYT_RS01305 at 298.382 kb on + strand, within BPHYT_RS01305 at 298.382 kb on + strand, within BPHYT_RS01305 at 298.538 kb on - strand, within BPHYT_RS01305 at 298.875 kb on + strand, within BPHYT_RS01305
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 0.1 remove 296,278 - +0.2 296,292 - -1.3 296,307 + -0.2 296,308 - -0.0 296,335 + +0.0 296,437 + -0.3 296,460 + -0.2 296,460 + -0.7 296,461 - -2.6 296,480 + +0.4 296,639 - BPHYT_RS01295 0.32 -1.3 296,747 + BPHYT_RS01295 0.48 -0.7 296,747 + BPHYT_RS01295 0.48 -0.1 296,748 - BPHYT_RS01295 0.48 -0.4 296,819 + BPHYT_RS01295 0.59 +0.4 296,826 + BPHYT_RS01295 0.60 -1.3 297,059 + -1.0 297,094 + +2.9 297,193 - -1.7 297,196 - -0.0 297,339 - BPHYT_RS01300 0.22 -2.1 297,542 + BPHYT_RS01300 0.51 -1.0 297,672 - BPHYT_RS01300 0.70 -0.7 297,672 - BPHYT_RS01300 0.70 +0.8 297,811 - -1.0 297,864 + -0.6 297,864 + +1.8 297,864 + +0.1 297,865 - +0.1 297,897 + -0.5 297,907 + +2.4 297,976 - +1.8 297,993 + +0.8 298,045 + -0.3 298,082 - +0.5 298,085 + +0.2 298,085 + +0.3 298,085 + +0.5 298,086 - +0.1 298,086 - -0.3 298,139 + BPHYT_RS01305 0.10 +2.4 298,171 - BPHYT_RS01305 0.13 +1.8 298,173 - BPHYT_RS01305 0.13 -0.3 298,173 - BPHYT_RS01305 0.13 +0.8 298,382 + BPHYT_RS01305 0.28 +0.6 298,382 + BPHYT_RS01305 0.28 +0.5 298,382 + BPHYT_RS01305 0.28 -0.8 298,382 + BPHYT_RS01305 0.28 +0.2 298,538 - BPHYT_RS01305 0.39 -0.4 298,875 + BPHYT_RS01305 0.63 -0.5
Or see this region's nucleotide sequence