Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27635

Experiment: 36hr PHYTO6 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27625 and BPHYT_RS27635 are separated by 604 nucleotidesBPHYT_RS27635 and BPHYT_RS27640 are separated by 431 nucleotides BPHYT_RS27625: BPHYT_RS27625 - transport-associated protein, at 1,766,577 to 1,767,227 _RS27625 BPHYT_RS27635: BPHYT_RS27635 - alcohol dehydrogenase, at 1,767,832 to 1,768,872 _RS27635 BPHYT_RS27640: BPHYT_RS27640 - acetoacetyl-CoA reductase, at 1,769,304 to 1,770,050 _RS27640 Position (kb) 1767 1768 1769Strain fitness (log2 ratio) -1 0 1 2at 1767.098 kb on - strand, within BPHYT_RS27625at 1767.249 kb on + strandat 1767.381 kb on - strandat 1767.592 kb on + strandat 1768.015 kb on - strand, within BPHYT_RS27635at 1768.073 kb on - strand, within BPHYT_RS27635at 1768.509 kb on - strand, within BPHYT_RS27635at 1768.509 kb on - strand, within BPHYT_RS27635at 1768.705 kb on - strand, within BPHYT_RS27635at 1768.856 kb on + strandat 1769.230 kb on + strandat 1769.422 kb on - strand, within BPHYT_RS27640at 1769.591 kb on - strand, within BPHYT_RS27640

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 0.1
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1,767,098 - BPHYT_RS27625 0.80 +0.9
1,767,249 + -0.9
1,767,381 - -0.2
1,767,592 + +0.1
1,768,015 - BPHYT_RS27635 0.18 -1.0
1,768,073 - BPHYT_RS27635 0.23 -1.6
1,768,509 - BPHYT_RS27635 0.65 +0.1
1,768,509 - BPHYT_RS27635 0.65 +2.4
1,768,705 - BPHYT_RS27635 0.84 -0.5
1,768,856 + -0.6
1,769,230 + -0.8
1,769,422 - BPHYT_RS27640 0.16 +1.1
1,769,591 - BPHYT_RS27640 0.38 -0.5

Or see this region's nucleotide sequence