Experiment: 36hr PHYTO6 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22940 and BPHYT_RS22945 are separated by 1 nucleotides BPHYT_RS22945 and BPHYT_RS22950 are separated by 53 nucleotides BPHYT_RS22950 and BPHYT_RS22955 are separated by 7 nucleotides BPHYT_RS22955 and BPHYT_RS22960 are separated by 63 nucleotides
BPHYT_RS22940: BPHYT_RS22940 - pilus assembly protein TadE, at 705,252 to 705,782
_RS22940
BPHYT_RS22945: BPHYT_RS22945 - pilus assembly protein TadE, at 705,784 to 706,413
_RS22945
BPHYT_RS22950: BPHYT_RS22950 - von Willebrand factor A, at 706,467 to 707,822
_RS22950
BPHYT_RS22955: BPHYT_RS22955 - isoprenylcysteine carboxyl methyltransferase, at 707,830 to 708,543
_RS22955
BPHYT_RS22960: BPHYT_RS22960 - peptidase, at 708,607 to 710,145
_RS22960
Position (kb)
706
707
708 Strain fitness (log2 ratio)
-2
-1
0
1 at 705.514 kb on - strand, within BPHYT_RS22940 at 705.630 kb on + strand, within BPHYT_RS22940 at 705.630 kb on + strand, within BPHYT_RS22940 at 705.887 kb on - strand, within BPHYT_RS22945 at 706.051 kb on + strand, within BPHYT_RS22945 at 706.052 kb on - strand, within BPHYT_RS22945 at 706.322 kb on + strand, within BPHYT_RS22945 at 706.393 kb on + strand at 706.599 kb on - strand at 706.934 kb on + strand, within BPHYT_RS22950 at 707.069 kb on - strand, within BPHYT_RS22950 at 707.130 kb on + strand, within BPHYT_RS22950 at 707.256 kb on - strand, within BPHYT_RS22950 at 707.353 kb on + strand, within BPHYT_RS22950 at 707.353 kb on + strand, within BPHYT_RS22950 at 707.526 kb on + strand, within BPHYT_RS22950 at 707.527 kb on - strand, within BPHYT_RS22950 at 707.527 kb on - strand, within BPHYT_RS22950 at 707.743 kb on - strand at 707.811 kb on - strand at 708.161 kb on - strand, within BPHYT_RS22955 at 708.325 kb on + strand, within BPHYT_RS22955 at 708.359 kb on - strand, within BPHYT_RS22955 at 708.548 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 0.1 remove 705,514 - BPHYT_RS22940 0.49 -1.5 705,630 + BPHYT_RS22940 0.71 +0.4 705,630 + BPHYT_RS22940 0.71 -0.4 705,887 - BPHYT_RS22945 0.16 -0.4 706,051 + BPHYT_RS22945 0.42 -0.4 706,052 - BPHYT_RS22945 0.43 +0.0 706,322 + BPHYT_RS22945 0.85 -0.1 706,393 + -1.0 706,599 - +0.4 706,934 + BPHYT_RS22950 0.34 -0.3 707,069 - BPHYT_RS22950 0.44 +0.1 707,130 + BPHYT_RS22950 0.49 +0.8 707,256 - BPHYT_RS22950 0.58 -0.1 707,353 + BPHYT_RS22950 0.65 -0.7 707,353 + BPHYT_RS22950 0.65 +0.2 707,526 + BPHYT_RS22950 0.78 -0.3 707,527 - BPHYT_RS22950 0.78 -1.0 707,527 - BPHYT_RS22950 0.78 +0.9 707,743 - -0.5 707,811 - -1.1 708,161 - BPHYT_RS22955 0.46 -1.4 708,325 + BPHYT_RS22955 0.69 -0.7 708,359 - BPHYT_RS22955 0.74 -1.9 708,548 - -0.2
Or see this region's nucleotide sequence