Experiment: 36hr PHYTO6 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20795 and BPHYT_RS20800 are separated by 95 nucleotides BPHYT_RS20800 and BPHYT_RS20805 are separated by 126 nucleotides
BPHYT_RS20795: BPHYT_RS20795 - sorbosone dehydrogenase, at 227,041 to 228,549
_RS20795
BPHYT_RS20800: BPHYT_RS20800 - alanine-phosphoribitol ligase, at 228,645 to 230,318
_RS20800
BPHYT_RS20805: BPHYT_RS20805 - LacI family transcriptional regulator, at 230,445 to 231,524
_RS20805
Position (kb) 228
229
230
231 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 227.742 kb on + strand, within BPHYT_RS20795 at 227.742 kb on + strand, within BPHYT_RS20795 at 227.743 kb on - strand, within BPHYT_RS20795 at 228.270 kb on + strand, within BPHYT_RS20795 at 228.491 kb on - strand at 228.491 kb on - strand at 228.622 kb on - strand at 228.648 kb on + strand at 228.648 kb on + strand at 228.648 kb on + strand at 228.648 kb on + strand at 228.654 kb on + strand at 228.654 kb on + strand at 228.765 kb on + strand at 228.767 kb on + strand at 228.943 kb on - strand, within BPHYT_RS20800 at 229.143 kb on + strand, within BPHYT_RS20800 at 229.143 kb on + strand, within BPHYT_RS20800 at 229.565 kb on + strand, within BPHYT_RS20800 at 229.565 kb on + strand, within BPHYT_RS20800 at 229.565 kb on + strand, within BPHYT_RS20800 at 229.791 kb on + strand, within BPHYT_RS20800 at 230.020 kb on - strand, within BPHYT_RS20800 at 230.369 kb on - strand at 230.817 kb on + strand, within BPHYT_RS20805 at 230.817 kb on + strand, within BPHYT_RS20805 at 230.818 kb on - strand, within BPHYT_RS20805
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 0.1 remove 227,742 + BPHYT_RS20795 0.46 -0.1 227,742 + BPHYT_RS20795 0.46 +0.8 227,743 - BPHYT_RS20795 0.47 -1.7 228,270 + BPHYT_RS20795 0.81 -0.4 228,491 - -0.8 228,491 - +1.6 228,622 - +1.2 228,648 + +0.4 228,648 + -0.9 228,648 + +0.6 228,648 + -0.0 228,654 + +3.5 228,654 + +1.4 228,765 + -0.3 228,767 + -0.2 228,943 - BPHYT_RS20800 0.18 +0.1 229,143 + BPHYT_RS20800 0.30 -2.1 229,143 + BPHYT_RS20800 0.30 +0.7 229,565 + BPHYT_RS20800 0.55 +0.5 229,565 + BPHYT_RS20800 0.55 +0.4 229,565 + BPHYT_RS20800 0.55 +0.8 229,791 + BPHYT_RS20800 0.68 +0.4 230,020 - BPHYT_RS20800 0.82 +0.8 230,369 - -0.6 230,817 + BPHYT_RS20805 0.34 -0.4 230,817 + BPHYT_RS20805 0.34 -0.1 230,818 - BPHYT_RS20805 0.35 -0.1
Or see this region's nucleotide sequence