Experiment: 36hr PHYTO6 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16620 and BPHYT_RS16625 are separated by 35 nucleotides BPHYT_RS16625 and BPHYT_RS16630 are separated by 177 nucleotides BPHYT_RS16630 and BPHYT_RS16635 are separated by 104 nucleotides
BPHYT_RS16620: BPHYT_RS16620 - holliday junction ATP-dependent DNA helicase RuvB, at 3,765,369 to 3,766,433
_RS16620
BPHYT_RS16625: BPHYT_RS16625 - hypothetical protein, at 3,766,469 to 3,767,410
_RS16625
BPHYT_RS16630: BPHYT_RS16630 - phosphoglycerate mutase, at 3,767,588 to 3,768,259
_RS16630
BPHYT_RS16635: BPHYT_RS16635 - D-aminoacyl-tRNA deacylase, at 3,768,364 to 3,768,822
_RS16635
Position (kb)
3766
3767
3768 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3766.641 kb on + strand, within BPHYT_RS16625 at 3766.641 kb on + strand, within BPHYT_RS16625 at 3766.641 kb on + strand, within BPHYT_RS16625 at 3766.642 kb on - strand, within BPHYT_RS16625 at 3766.798 kb on + strand, within BPHYT_RS16625 at 3766.798 kb on + strand, within BPHYT_RS16625 at 3766.983 kb on - strand, within BPHYT_RS16625 at 3766.983 kb on - strand, within BPHYT_RS16625 at 3766.983 kb on - strand, within BPHYT_RS16625 at 3767.099 kb on + strand, within BPHYT_RS16625 at 3767.099 kb on + strand, within BPHYT_RS16625 at 3767.099 kb on + strand, within BPHYT_RS16625 at 3767.099 kb on + strand, within BPHYT_RS16625 at 3767.100 kb on - strand, within BPHYT_RS16625 at 3767.100 kb on - strand, within BPHYT_RS16625 at 3767.187 kb on + strand, within BPHYT_RS16625 at 3767.188 kb on - strand, within BPHYT_RS16625 at 3767.276 kb on + strand, within BPHYT_RS16625 at 3767.395 kb on - strand at 3767.409 kb on - strand at 3767.479 kb on - strand at 3767.727 kb on + strand, within BPHYT_RS16630 at 3767.727 kb on + strand, within BPHYT_RS16630 at 3767.727 kb on + strand, within BPHYT_RS16630 at 3767.728 kb on - strand, within BPHYT_RS16630 at 3767.732 kb on - strand, within BPHYT_RS16630 at 3767.866 kb on - strand, within BPHYT_RS16630 at 3767.866 kb on - strand, within BPHYT_RS16630 at 3767.866 kb on - strand, within BPHYT_RS16630 at 3767.932 kb on - strand, within BPHYT_RS16630 at 3767.932 kb on - strand, within BPHYT_RS16630 at 3767.932 kb on - strand, within BPHYT_RS16630 at 3767.965 kb on + strand, within BPHYT_RS16630 at 3767.989 kb on - strand, within BPHYT_RS16630
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 0.1 remove 3,766,641 + BPHYT_RS16625 0.18 +0.1 3,766,641 + BPHYT_RS16625 0.18 +1.1 3,766,641 + BPHYT_RS16625 0.18 -0.5 3,766,642 - BPHYT_RS16625 0.18 -0.1 3,766,798 + BPHYT_RS16625 0.35 -0.8 3,766,798 + BPHYT_RS16625 0.35 -1.7 3,766,983 - BPHYT_RS16625 0.55 +1.2 3,766,983 - BPHYT_RS16625 0.55 -1.7 3,766,983 - BPHYT_RS16625 0.55 -1.0 3,767,099 + BPHYT_RS16625 0.67 +0.1 3,767,099 + BPHYT_RS16625 0.67 -0.6 3,767,099 + BPHYT_RS16625 0.67 -0.2 3,767,099 + BPHYT_RS16625 0.67 -0.1 3,767,100 - BPHYT_RS16625 0.67 +0.4 3,767,100 - BPHYT_RS16625 0.67 -0.4 3,767,187 + BPHYT_RS16625 0.76 -0.3 3,767,188 - BPHYT_RS16625 0.76 -1.4 3,767,276 + BPHYT_RS16625 0.86 +0.1 3,767,395 - +0.5 3,767,409 - -0.1 3,767,479 - +0.2 3,767,727 + BPHYT_RS16630 0.21 -0.1 3,767,727 + BPHYT_RS16630 0.21 -1.5 3,767,727 + BPHYT_RS16630 0.21 +0.6 3,767,728 - BPHYT_RS16630 0.21 -0.4 3,767,732 - BPHYT_RS16630 0.21 -0.1 3,767,866 - BPHYT_RS16630 0.41 +0.2 3,767,866 - BPHYT_RS16630 0.41 +3.6 3,767,866 - BPHYT_RS16630 0.41 +0.5 3,767,932 - BPHYT_RS16630 0.51 +0.5 3,767,932 - BPHYT_RS16630 0.51 -1.7 3,767,932 - BPHYT_RS16630 0.51 +0.4 3,767,965 + BPHYT_RS16630 0.56 -0.3 3,767,989 - BPHYT_RS16630 0.60 +0.2
Or see this region's nucleotide sequence