Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS36115 and BPHYT_RS36120 are separated by 82 nucleotides BPHYT_RS36120 and BPHYT_RS36125 are separated by 244 nucleotides BPHYT_RS36125 and BPHYT_RS36130 are separated by 44 nucleotides BPHYT_RS36130 and BPHYT_RS36135 are separated by 662 nucleotides
BPHYT_RS36115: BPHYT_RS36115 - hypothetical protein, at 43,394 to 43,693
_RS36115
BPHYT_RS36120: BPHYT_RS36120 - hypothetical protein, at 43,776 to 44,009
_RS36120
BPHYT_RS36125: BPHYT_RS36125 - hypothetical protein, at 44,254 to 44,436
_RS36125
BPHYT_RS36130: BPHYT_RS36130 - hypothetical protein, at 44,481 to 44,753
_RS36130
BPHYT_RS36135: BPHYT_RS36135 - hypothetical protein, at 45,416 to 45,631
_RS36135
Position (kb)
44
45 Strain fitness (log2 ratio)
-2
-1
0
1 at 44.113 kb on + strand at 44.191 kb on + strand at 44.191 kb on + strand at 44.192 kb on - strand at 44.214 kb on - strand at 44.214 kb on - strand at 44.419 kb on + strand at 44.641 kb on - strand, within BPHYT_RS36130 at 44.736 kb on + strand at 44.784 kb on + strand at 44.912 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 44,113 + -0.9 44,191 + -2.1 44,191 + +0.1 44,192 - -1.2 44,214 - -0.2 44,214 - -0.7 44,419 + +0.1 44,641 - BPHYT_RS36130 0.59 -1.9 44,736 + -0.3 44,784 + +1.4 44,912 - -0.7
Or see this region's nucleotide sequence