Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35025 and BPHYT_RS35030 are separated by 137 nucleotides BPHYT_RS35030 and BPHYT_RS35035 are separated by 176 nucleotides BPHYT_RS35035 and BPHYT_RS35040 are separated by 351 nucleotides
BPHYT_RS35025: BPHYT_RS35025 - sarcosine oxidase, at 3,461,407 to 3,462,510
_RS35025
BPHYT_RS35030: BPHYT_RS35030 - hypothetical protein, at 3,462,648 to 3,462,851
_RS35030
BPHYT_RS35035: BPHYT_RS35035 - membrane protein, at 3,463,028 to 3,464,089
_RS35035
BPHYT_RS35040: BPHYT_RS35040 - lipocalin, at 3,464,441 to 3,465,037
_RS35040
Position (kb)
3463
3464
3465 Strain fitness (log2 ratio)
-1
0
1 at 3462.057 kb on + strand, within BPHYT_RS35025 at 3462.270 kb on - strand, within BPHYT_RS35025 at 3462.310 kb on - strand, within BPHYT_RS35025 at 3462.356 kb on + strand, within BPHYT_RS35025 at 3462.687 kb on + strand, within BPHYT_RS35030 at 3462.716 kb on - strand, within BPHYT_RS35030 at 3462.716 kb on - strand, within BPHYT_RS35030 at 3462.763 kb on + strand, within BPHYT_RS35030 at 3462.764 kb on - strand, within BPHYT_RS35030 at 3462.820 kb on - strand, within BPHYT_RS35030 at 3462.828 kb on + strand, within BPHYT_RS35030 at 3462.829 kb on - strand, within BPHYT_RS35030 at 3462.851 kb on + strand at 3462.852 kb on - strand at 3462.852 kb on - strand at 3462.890 kb on - strand at 3463.341 kb on + strand, within BPHYT_RS35035 at 3463.341 kb on + strand, within BPHYT_RS35035 at 3463.352 kb on - strand, within BPHYT_RS35035 at 3463.518 kb on - strand, within BPHYT_RS35035 at 3463.518 kb on - strand, within BPHYT_RS35035 at 3464.128 kb on - strand at 3464.153 kb on + strand at 3464.382 kb on - strand at 3464.384 kb on + strand at 3464.384 kb on + strand at 3464.384 kb on + strand at 3464.385 kb on - strand at 3464.385 kb on - strand at 3464.436 kb on - strand at 3464.574 kb on - strand, within BPHYT_RS35040 at 3464.574 kb on - strand, within BPHYT_RS35040 at 3464.685 kb on - strand, within BPHYT_RS35040 at 3464.687 kb on + strand, within BPHYT_RS35040 at 3464.727 kb on - strand, within BPHYT_RS35040 at 3464.820 kb on - strand, within BPHYT_RS35040 at 3465.077 kb on - strand at 3465.077 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 3,462,057 + BPHYT_RS35025 0.59 +1.5 3,462,270 - BPHYT_RS35025 0.78 -0.3 3,462,310 - BPHYT_RS35025 0.82 -0.3 3,462,356 + BPHYT_RS35025 0.86 +0.3 3,462,687 + BPHYT_RS35030 0.19 +0.1 3,462,716 - BPHYT_RS35030 0.33 +0.2 3,462,716 - BPHYT_RS35030 0.33 -0.6 3,462,763 + BPHYT_RS35030 0.56 -1.6 3,462,764 - BPHYT_RS35030 0.57 -0.2 3,462,820 - BPHYT_RS35030 0.84 -1.0 3,462,828 + BPHYT_RS35030 0.88 +0.5 3,462,829 - BPHYT_RS35030 0.89 -0.3 3,462,851 + +0.3 3,462,852 - -0.1 3,462,852 - +0.4 3,462,890 - +1.3 3,463,341 + BPHYT_RS35035 0.29 +0.5 3,463,341 + BPHYT_RS35035 0.29 +0.1 3,463,352 - BPHYT_RS35035 0.31 -1.2 3,463,518 - BPHYT_RS35035 0.46 -0.2 3,463,518 - BPHYT_RS35035 0.46 -0.1 3,464,128 - +0.2 3,464,153 + -0.9 3,464,382 - -1.7 3,464,384 + -0.3 3,464,384 + +0.1 3,464,384 + -0.5 3,464,385 - -0.7 3,464,385 - -1.3 3,464,436 - -1.6 3,464,574 - BPHYT_RS35040 0.22 -1.3 3,464,574 - BPHYT_RS35040 0.22 +1.5 3,464,685 - BPHYT_RS35040 0.41 -1.0 3,464,687 + BPHYT_RS35040 0.41 -0.0 3,464,727 - BPHYT_RS35040 0.48 -0.3 3,464,820 - BPHYT_RS35040 0.63 -0.2 3,465,077 - -1.3 3,465,077 - +0.0
Or see this region's nucleotide sequence