Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31175 and BPHYT_RS31180 are separated by 6 nucleotides BPHYT_RS31180 and BPHYT_RS31185 are separated by 124 nucleotides BPHYT_RS31185 and BPHYT_RS31190 are separated by 141 nucleotides
BPHYT_RS31175: BPHYT_RS31175 - RNA polymerase sigma70 factor, at 2,552,511 to 2,553,785
_RS31175
BPHYT_RS31180: BPHYT_RS31180 - DGPFAETKE family protein, at 2,553,792 to 2,554,163
_RS31180
BPHYT_RS31185: BPHYT_RS31185 - LysR family transcriptional regulator, at 2,554,288 to 2,555,199
_RS31185
BPHYT_RS31190: BPHYT_RS31190 - ABC transporter permease, at 2,555,341 to 2,556,312
_RS31190
Position (kb)
2554
2555
2556 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2554.142 kb on - strand at 2554.157 kb on - strand at 2554.194 kb on - strand at 2554.198 kb on + strand at 2554.198 kb on + strand at 2554.199 kb on - strand at 2554.390 kb on + strand, within BPHYT_RS31185 at 2554.390 kb on + strand, within BPHYT_RS31185 at 2554.687 kb on + strand, within BPHYT_RS31185 at 2554.809 kb on - strand, within BPHYT_RS31185 at 2554.809 kb on - strand, within BPHYT_RS31185 at 2554.809 kb on - strand, within BPHYT_RS31185 at 2555.220 kb on - strand at 2555.220 kb on - strand at 2555.220 kb on - strand at 2555.220 kb on - strand at 2555.408 kb on + strand at 2555.530 kb on + strand, within BPHYT_RS31190 at 2555.531 kb on - strand, within BPHYT_RS31190 at 2555.531 kb on - strand, within BPHYT_RS31190 at 2555.670 kb on - strand, within BPHYT_RS31190 at 2555.670 kb on - strand, within BPHYT_RS31190 at 2555.996 kb on - strand, within BPHYT_RS31190
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 2,554,142 - +0.0 2,554,157 - -0.4 2,554,194 - +2.8 2,554,198 + +0.6 2,554,198 + -1.1 2,554,199 - +0.8 2,554,390 + BPHYT_RS31185 0.11 -1.8 2,554,390 + BPHYT_RS31185 0.11 -1.0 2,554,687 + BPHYT_RS31185 0.44 -0.4 2,554,809 - BPHYT_RS31185 0.57 +0.0 2,554,809 - BPHYT_RS31185 0.57 -1.9 2,554,809 - BPHYT_RS31185 0.57 -1.3 2,555,220 - -1.3 2,555,220 - -0.5 2,555,220 - -0.1 2,555,220 - -1.6 2,555,408 + -0.8 2,555,530 + BPHYT_RS31190 0.19 -1.8 2,555,531 - BPHYT_RS31190 0.20 -1.9 2,555,531 - BPHYT_RS31190 0.20 -1.3 2,555,670 - BPHYT_RS31190 0.34 -0.0 2,555,670 - BPHYT_RS31190 0.34 +3.6 2,555,996 - BPHYT_RS31190 0.67 +0.3
Or see this region's nucleotide sequence