Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31020 and BPHYT_RS31025 overlap by 4 nucleotides BPHYT_RS31025 and BPHYT_RS31030 are separated by 114 nucleotides BPHYT_RS31030 and BPHYT_RS31035 are separated by 57 nucleotides BPHYT_RS31035 and BPHYT_RS31040 are separated by 2 nucleotides
BPHYT_RS31020: BPHYT_RS31020 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 2,514,001 to 2,514,555
_RS31020
BPHYT_RS31025: BPHYT_RS31025 - 2-dehydro-3-deoxyphosphooctonate aldolase, at 2,514,552 to 2,515,412
_RS31025
BPHYT_RS31030: BPHYT_RS31030 - tyrosine protein kinase, at 2,515,527 to 2,517,752
_RS31030
BPHYT_RS31035: BPHYT_RS31035 - protein-tyrosine-phosphatase, at 2,517,810 to 2,518,247
_RS31035
BPHYT_RS31040: BPHYT_RS31040 - sugar transporter, at 2,518,250 to 2,519,407
_RS31040
Position (kb)
2515
2516
2517
2518 Strain fitness (log2 ratio)
-2
-1
0
1 at 2515.298 kb on - strand, within BPHYT_RS31025 at 2515.298 kb on - strand, within BPHYT_RS31025 at 2515.571 kb on - strand at 2516.262 kb on + strand, within BPHYT_RS31030 at 2516.263 kb on - strand, within BPHYT_RS31030 at 2516.556 kb on - strand, within BPHYT_RS31030 at 2516.945 kb on - strand, within BPHYT_RS31030 at 2517.729 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 2,515,298 - BPHYT_RS31025 0.87 -1.0 2,515,298 - BPHYT_RS31025 0.87 -0.7 2,515,571 - -2.3 2,516,262 + BPHYT_RS31030 0.33 +0.8 2,516,263 - BPHYT_RS31030 0.33 +0.1 2,516,556 - BPHYT_RS31030 0.46 -0.8 2,516,945 - BPHYT_RS31030 0.64 -0.2 2,517,729 - +0.6
Or see this region's nucleotide sequence