Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28830

Experiment: 36hr PHYTO6 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28825 and BPHYT_RS28830 are separated by 260 nucleotidesBPHYT_RS28830 and BPHYT_RS28835 are separated by 279 nucleotides BPHYT_RS28825: BPHYT_RS28825 - methylmalonate-semialdehyde dehydrogenase, at 2,017,055 to 2,018,578 _RS28825 BPHYT_RS28830: BPHYT_RS28830 - LysR family transcriptional regulator, at 2,018,839 to 2,019,795 _RS28830 BPHYT_RS28835: BPHYT_RS28835 - 3-(2-hydroxyphenyl) propionic acid transporter, at 2,020,075 to 2,021,481 _RS28835 Position (kb) 2018 2019 2020Strain fitness (log2 ratio) -1 0 1at 2017.851 kb on + strand, within BPHYT_RS28825at 2018.580 kb on + strandat 2018.628 kb on + strandat 2018.981 kb on + strand, within BPHYT_RS28830at 2020.168 kb on + strandat 2020.168 kb on + strandat 2020.168 kb on + strandat 2020.183 kb on + strandat 2020.543 kb on + strand, within BPHYT_RS28835at 2020.543 kb on + strand, within BPHYT_RS28835at 2020.544 kb on - strand, within BPHYT_RS28835at 2020.619 kb on - strand, within BPHYT_RS28835

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10
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2,017,851 + BPHYT_RS28825 0.52 +0.5
2,018,580 + -1.3
2,018,628 + +1.5
2,018,981 + BPHYT_RS28830 0.15 -0.9
2,020,168 + +0.7
2,020,168 + +0.2
2,020,168 + -0.3
2,020,183 + +0.4
2,020,543 + BPHYT_RS28835 0.33 +0.2
2,020,543 + BPHYT_RS28835 0.33 -0.9
2,020,544 - BPHYT_RS28835 0.33 +0.2
2,020,619 - BPHYT_RS28835 0.39 -0.3

Or see this region's nucleotide sequence