Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28515

Experiment: 36hr PHYTO6 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28510 and BPHYT_RS28515 overlap by 14 nucleotidesBPHYT_RS28515 and BPHYT_RS28520 are separated by 499 nucleotides BPHYT_RS28510: BPHYT_RS28510 - dioxygenase, at 1,950,820 to 1,952,298 _RS28510 BPHYT_RS28515: BPHYT_RS28515 - mannonate dehydratase, at 1,952,285 to 1,953,337 _RS28515 BPHYT_RS28520: BPHYT_RS28520 - hypothetical protein, at 1,953,837 to 1,955,438 _RS28520 Position (kb) 1952 1953 1954Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1951.377 kb on - strand, within BPHYT_RS28510at 1951.427 kb on + strand, within BPHYT_RS28510at 1951.640 kb on - strand, within BPHYT_RS28510at 1951.893 kb on - strand, within BPHYT_RS28510at 1952.154 kb on - strandat 1953.185 kb on + strand, within BPHYT_RS28515at 1953.185 kb on + strand, within BPHYT_RS28515at 1953.186 kb on - strand, within BPHYT_RS28515at 1953.247 kb on - strandat 1953.291 kb on + strandat 1953.459 kb on + strandat 1953.460 kb on - strandat 1953.503 kb on + strandat 1953.528 kb on - strandat 1953.552 kb on - strandat 1953.657 kb on + strandat 1953.658 kb on - strandat 1953.669 kb on + strandat 1953.669 kb on + strandat 1953.669 kb on + strandat 1953.883 kb on + strandat 1954.096 kb on + strand, within BPHYT_RS28520at 1954.096 kb on + strand, within BPHYT_RS28520

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10
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1,951,377 - BPHYT_RS28510 0.38 -2.2
1,951,427 + BPHYT_RS28510 0.41 +0.1
1,951,640 - BPHYT_RS28510 0.55 -1.7
1,951,893 - BPHYT_RS28510 0.73 -0.7
1,952,154 - +1.2
1,953,185 + BPHYT_RS28515 0.85 +0.1
1,953,185 + BPHYT_RS28515 0.85 +2.8
1,953,186 - BPHYT_RS28515 0.86 -1.0
1,953,247 - -1.6
1,953,291 + -1.0
1,953,459 + +0.3
1,953,460 - -0.5
1,953,503 + +0.2
1,953,528 - +2.6
1,953,552 - -0.1
1,953,657 + +0.2
1,953,658 - +1.8
1,953,669 + -2.3
1,953,669 + -0.8
1,953,669 + +2.0
1,953,883 + +0.6
1,954,096 + BPHYT_RS28520 0.16 -0.0
1,954,096 + BPHYT_RS28520 0.16 -1.8

Or see this region's nucleotide sequence