Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28095 and BPHYT_RS28100 are separated by 25 nucleotides BPHYT_RS28100 and BPHYT_RS28105 are separated by 55 nucleotides BPHYT_RS28105 and BPHYT_RS28110 overlap by 28 nucleotides BPHYT_RS28110 and BPHYT_RS28115 are separated by 19 nucleotides BPHYT_RS28115 and BPHYT_RS28120 are separated by 578 nucleotides
BPHYT_RS28095: BPHYT_RS28095 - membrane protein, at 1,866,268 to 1,866,462
_RS28095
BPHYT_RS28100: BPHYT_RS28100 - hypothetical protein, at 1,866,488 to 1,866,682
_RS28100
BPHYT_RS28105: BPHYT_RS28105 - hypothetical protein, at 1,866,738 to 1,867,382
_RS28105
BPHYT_RS28110: BPHYT_RS28110 - hypothetical protein, at 1,867,355 to 1,867,555
_RS28110
BPHYT_RS28115: BPHYT_RS28115 - hypothetical protein, at 1,867,575 to 1,867,793
_RS28115
BPHYT_RS28120: BPHYT_RS28120 - hypothetical protein, at 1,868,372 to 1,868,653
_RS28120
Position (kb) 1867
1868 Strain fitness (log2 ratio) -1
0
1
2 at 1866.575 kb on - strand, within BPHYT_RS28100 at 1866.591 kb on + strand, within BPHYT_RS28100 at 1866.648 kb on + strand, within BPHYT_RS28100 at 1866.649 kb on - strand, within BPHYT_RS28100 at 1866.773 kb on - strand at 1866.811 kb on + strand, within BPHYT_RS28105 at 1866.935 kb on - strand, within BPHYT_RS28105 at 1867.425 kb on + strand, within BPHYT_RS28110 at 1867.558 kb on - strand at 1867.558 kb on - strand at 1867.662 kb on + strand, within BPHYT_RS28115 at 1867.965 kb on + strand at 1867.975 kb on + strand at 1868.352 kb on + strand at 1868.355 kb on + strand at 1868.393 kb on + strand at 1868.393 kb on + strand at 1868.394 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 1,866,575 - BPHYT_RS28100 0.45 +0.7 1,866,591 + BPHYT_RS28100 0.53 +0.6 1,866,648 + BPHYT_RS28100 0.82 +0.5 1,866,649 - BPHYT_RS28100 0.83 -0.3 1,866,773 - +0.7 1,866,811 + BPHYT_RS28105 0.11 -0.8 1,866,935 - BPHYT_RS28105 0.31 +1.8 1,867,425 + BPHYT_RS28110 0.35 -1.3 1,867,558 - +0.0 1,867,558 - -1.4 1,867,662 + BPHYT_RS28115 0.40 -0.6 1,867,965 + -1.6 1,867,975 + -0.7 1,868,352 + +0.1 1,868,355 + +0.2 1,868,393 + -0.6 1,868,393 + +0.9 1,868,394 - -0.4
Or see this region's nucleotide sequence