Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27345

Experiment: 36hr PHYTO6 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27335 and BPHYT_RS27340 overlap by 4 nucleotidesBPHYT_RS27340 and BPHYT_RS27345 overlap by 4 nucleotidesBPHYT_RS27345 and BPHYT_RS27350 are separated by 184 nucleotides BPHYT_RS27335: BPHYT_RS27335 - cytochrome C, at 1,707,461 to 1,708,738 _RS27335 BPHYT_RS27340: BPHYT_RS27340 - (2Fe-2S)-binding protein, at 1,708,735 to 1,709,247 _RS27340 BPHYT_RS27345: BPHYT_RS27345 - aldehyde dehydrogenase, at 1,709,244 to 1,711,508 _RS27345 BPHYT_RS27350: BPHYT_RS27350 - LysR family transcriptional regulator, at 1,711,693 to 1,712,598 _RS27350 Position (kb) 1709 1710 1711 1712Strain fitness (log2 ratio) -1 0 1at 1708.593 kb on - strand, within BPHYT_RS27335at 1708.616 kb on + strandat 1708.802 kb on - strand, within BPHYT_RS27340at 1710.675 kb on - strand, within BPHYT_RS27345at 1711.072 kb on - strand, within BPHYT_RS27345at 1711.268 kb on + strand, within BPHYT_RS27345at 1711.541 kb on + strandat 1711.600 kb on + strandat 1711.601 kb on - strandat 1711.761 kb on - strandat 1712.028 kb on - strand, within BPHYT_RS27350at 1712.101 kb on + strand, within BPHYT_RS27350at 1712.101 kb on + strand, within BPHYT_RS27350at 1712.295 kb on + strand, within BPHYT_RS27350

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10
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1,708,593 - BPHYT_RS27335 0.89 -0.3
1,708,616 + -1.5
1,708,802 - BPHYT_RS27340 0.13 +0.6
1,710,675 - BPHYT_RS27345 0.63 -1.3
1,711,072 - BPHYT_RS27345 0.81 -1.3
1,711,268 + BPHYT_RS27345 0.89 +1.0
1,711,541 + +1.0
1,711,600 + -0.0
1,711,601 - -0.1
1,711,761 - +1.3
1,712,028 - BPHYT_RS27350 0.37 +0.3
1,712,101 + BPHYT_RS27350 0.45 +1.3
1,712,101 + BPHYT_RS27350 0.45 +0.1
1,712,295 + BPHYT_RS27350 0.66 +0.4

Or see this region's nucleotide sequence