Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26680 and BPHYT_RS26685 overlap by 8 nucleotides BPHYT_RS26685 and BPHYT_RS26690 are separated by 0 nucleotides BPHYT_RS26690 and BPHYT_RS26695 overlap by 13 nucleotides
BPHYT_RS26680: BPHYT_RS26680 - oxidoreductase, at 1,554,444 to 1,555,886
_RS26680
BPHYT_RS26685: BPHYT_RS26685 - precorrin-8X methylmutase, at 1,555,879 to 1,556,505
_RS26685
BPHYT_RS26690: BPHYT_RS26690 - precorrin-2 C20-methyltransferase, at 1,556,506 to 1,557,237
_RS26690
BPHYT_RS26695: BPHYT_RS26695 - cobalamin biosynthesis protein CbiG, at 1,557,225 to 1,558,937
_RS26695
Position (kb) 1556
1557
1558 Strain fitness (log2 ratio) -2
-1
0
1 at 1555.889 kb on - strand at 1555.913 kb on - strand at 1556.172 kb on + strand, within BPHYT_RS26685 at 1556.313 kb on + strand, within BPHYT_RS26685 at 1556.611 kb on + strand, within BPHYT_RS26690 at 1556.854 kb on + strand, within BPHYT_RS26690 at 1556.855 kb on - strand, within BPHYT_RS26690 at 1556.860 kb on + strand, within BPHYT_RS26690 at 1556.860 kb on + strand, within BPHYT_RS26690 at 1556.860 kb on + strand, within BPHYT_RS26690
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 1,555,889 - -1.0 1,555,913 - -1.2 1,556,172 + BPHYT_RS26685 0.47 +0.3 1,556,313 + BPHYT_RS26685 0.69 +1.7 1,556,611 + BPHYT_RS26690 0.14 -1.8 1,556,854 + BPHYT_RS26690 0.48 +0.3 1,556,855 - BPHYT_RS26690 0.48 +0.6 1,556,860 + BPHYT_RS26690 0.48 +1.0 1,556,860 + BPHYT_RS26690 0.48 -1.3 1,556,860 + BPHYT_RS26690 0.48 +0.6
Or see this region's nucleotide sequence