Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26400 and BPHYT_RS26405 are separated by 318 nucleotides BPHYT_RS26405 and BPHYT_RS26410 are separated by 82 nucleotides BPHYT_RS26410 and BPHYT_RS26415 are separated by 155 nucleotides
BPHYT_RS26400: BPHYT_RS26400 - sodium:solute symporter, at 1,495,203 to 1,496,675
_RS26400
BPHYT_RS26405: BPHYT_RS26405 - glycogen synthase, at 1,496,994 to 1,498,583
_RS26405
BPHYT_RS26410: BPHYT_RS26410 - hypothetical protein, at 1,498,666 to 1,499,055
_RS26410
BPHYT_RS26415: BPHYT_RS26415 - glutathione S-transferase, at 1,499,211 to 1,499,873
_RS26415
Position (kb) 1496
1497
1498
1499 Strain fitness (log2 ratio) -2
-1
0
1 at 1496.247 kb on + strand, within BPHYT_RS26400 at 1496.573 kb on + strand at 1496.696 kb on + strand at 1496.828 kb on - strand at 1497.795 kb on - strand, within BPHYT_RS26405 at 1497.795 kb on - strand, within BPHYT_RS26405 at 1497.807 kb on + strand, within BPHYT_RS26405 at 1497.951 kb on - strand, within BPHYT_RS26405 at 1498.243 kb on - strand, within BPHYT_RS26405 at 1498.428 kb on + strand at 1498.628 kb on + strand at 1498.629 kb on - strand at 1498.713 kb on + strand, within BPHYT_RS26410 at 1498.730 kb on - strand, within BPHYT_RS26410 at 1498.732 kb on - strand, within BPHYT_RS26410 at 1499.173 kb on - strand at 1499.232 kb on + strand at 1499.241 kb on + strand at 1499.523 kb on + strand, within BPHYT_RS26415
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 1,496,247 + BPHYT_RS26400 0.71 -2.3 1,496,573 + -0.2 1,496,696 + +0.3 1,496,828 - +0.9 1,497,795 - BPHYT_RS26405 0.50 +0.8 1,497,795 - BPHYT_RS26405 0.50 -1.1 1,497,807 + BPHYT_RS26405 0.51 -1.1 1,497,951 - BPHYT_RS26405 0.60 -1.3 1,498,243 - BPHYT_RS26405 0.79 -0.4 1,498,428 + +0.9 1,498,628 + -1.4 1,498,629 - -0.0 1,498,713 + BPHYT_RS26410 0.12 +0.2 1,498,730 - BPHYT_RS26410 0.16 -0.1 1,498,732 - BPHYT_RS26410 0.17 -0.4 1,499,173 - -0.9 1,499,232 + -0.4 1,499,241 + +0.0 1,499,523 + BPHYT_RS26415 0.47 +0.4
Or see this region's nucleotide sequence