Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24725 and BPHYT_RS24730 are separated by 207 nucleotides BPHYT_RS24730 and BPHYT_RS24735 are separated by 93 nucleotides BPHYT_RS24735 and BPHYT_RS24740 overlap by 4 nucleotides
BPHYT_RS24725: BPHYT_RS24725 - NmrA family transcriptional regulator, at 1,113,598 to 1,114,521
_RS24725
BPHYT_RS24730: BPHYT_RS24730 - AraC family transcriptional regulator, at 1,114,729 to 1,115,685
_RS24730
BPHYT_RS24735: BPHYT_RS24735 - transcriptional regulator, at 1,115,779 to 1,116,606
_RS24735
BPHYT_RS24740: BPHYT_RS24740 - RNA polymerase subunit sigma-24, at 1,116,603 to 1,117,121
_RS24740
Position (kb)
1114
1115
1116 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1113.911 kb on - strand, within BPHYT_RS24725 at 1114.066 kb on + strand, within BPHYT_RS24725 at 1114.067 kb on - strand, within BPHYT_RS24725 at 1114.086 kb on + strand, within BPHYT_RS24725 at 1114.087 kb on - strand, within BPHYT_RS24725 at 1114.558 kb on + strand at 1114.624 kb on + strand at 1114.656 kb on - strand at 1114.659 kb on + strand at 1114.884 kb on + strand, within BPHYT_RS24730 at 1114.885 kb on - strand, within BPHYT_RS24730 at 1114.900 kb on + strand, within BPHYT_RS24730 at 1115.242 kb on - strand, within BPHYT_RS24730 at 1115.634 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 1,113,911 - BPHYT_RS24725 0.34 -0.0 1,114,066 + BPHYT_RS24725 0.51 -0.4 1,114,067 - BPHYT_RS24725 0.51 +0.4 1,114,086 + BPHYT_RS24725 0.53 -0.8 1,114,087 - BPHYT_RS24725 0.53 -1.1 1,114,558 + -1.3 1,114,624 + -0.9 1,114,656 - +2.7 1,114,659 + -1.8 1,114,884 + BPHYT_RS24730 0.16 -0.8 1,114,885 - BPHYT_RS24730 0.16 -0.8 1,114,900 + BPHYT_RS24730 0.18 -0.7 1,115,242 - BPHYT_RS24730 0.54 -0.8 1,115,634 + -1.3
Or see this region's nucleotide sequence