Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22770 and BPHYT_RS22775 are separated by 145 nucleotides BPHYT_RS22775 and BPHYT_RS22780 are separated by 10 nucleotides
BPHYT_RS22770: BPHYT_RS22770 - sugar ABC transporter substrate-binding protein, at 666,876 to 668,129
_RS22770
BPHYT_RS22775: BPHYT_RS22775 - sugar ABC transporter permease, at 668,275 to 669,252
_RS22775
BPHYT_RS22780: BPHYT_RS22780 - sugar ABC transporter permease, at 669,263 to 670,114
_RS22780
Position (kb) 668
669
670 Strain fitness (log2 ratio) -2
-1
0
1
2 at 667.564 kb on - strand, within BPHYT_RS22770 at 667.799 kb on + strand, within BPHYT_RS22770 at 667.996 kb on - strand, within BPHYT_RS22770 at 667.996 kb on - strand, within BPHYT_RS22770 at 668.118 kb on + strand at 668.187 kb on - strand at 668.187 kb on - strand at 668.200 kb on + strand at 668.212 kb on + strand at 668.213 kb on - strand at 668.471 kb on - strand, within BPHYT_RS22775 at 668.590 kb on + strand, within BPHYT_RS22775 at 668.668 kb on + strand, within BPHYT_RS22775 at 668.902 kb on + strand, within BPHYT_RS22775 at 669.124 kb on + strand, within BPHYT_RS22775 at 669.148 kb on + strand, within BPHYT_RS22775 at 669.488 kb on + strand, within BPHYT_RS22780 at 669.489 kb on - strand, within BPHYT_RS22780 at 670.233 kb on + strand at 670.234 kb on - strand at 670.234 kb on - strand at 670.237 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 667,564 - BPHYT_RS22770 0.55 +0.2 667,799 + BPHYT_RS22770 0.74 -1.4 667,996 - BPHYT_RS22770 0.89 +0.2 667,996 - BPHYT_RS22770 0.89 -0.2 668,118 + -0.1 668,187 - -1.6 668,187 - +1.0 668,200 + -0.2 668,212 + -0.1 668,213 - +0.0 668,471 - BPHYT_RS22775 0.20 -1.9 668,590 + BPHYT_RS22775 0.32 -0.5 668,668 + BPHYT_RS22775 0.40 -1.5 668,902 + BPHYT_RS22775 0.64 -1.1 669,124 + BPHYT_RS22775 0.87 -1.4 669,148 + BPHYT_RS22775 0.89 -0.1 669,488 + BPHYT_RS22780 0.26 +0.3 669,489 - BPHYT_RS22780 0.27 -0.3 670,233 + +0.7 670,234 - +1.3 670,234 - -0.1 670,237 + +1.8
Or see this region's nucleotide sequence