Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22725 and BPHYT_RS22730 are separated by 52 nucleotides BPHYT_RS22730 and BPHYT_RS22735 are separated by 30 nucleotides BPHYT_RS22735 and BPHYT_RS22740 are separated by 19 nucleotides
BPHYT_RS22725: BPHYT_RS22725 - glycosyl transferase family 51, at 653,652 to 656,732
_RS22725
BPHYT_RS22730: BPHYT_RS22730 - serine/threonine kinase, at 656,785 to 658,011
_RS22730
BPHYT_RS22735: BPHYT_RS22735 - sugar ABC transporter ATP-binding protein, at 658,042 to 658,839
_RS22735
BPHYT_RS22740: BPHYT_RS22740 - ABC transporter permease, at 658,859 to 659,845
_RS22740
Position (kb) 656
657
658
659 Strain fitness (log2 ratio) -2
-1
0
1
2 at 655.851 kb on - strand, within BPHYT_RS22725 at 655.858 kb on - strand, within BPHYT_RS22725 at 655.904 kb on + strand, within BPHYT_RS22725 at 656.601 kb on + strand at 656.711 kb on + strand at 656.846 kb on + strand at 656.847 kb on - strand at 657.527 kb on - strand, within BPHYT_RS22730 at 657.734 kb on + strand, within BPHYT_RS22730 at 658.562 kb on - strand, within BPHYT_RS22735 at 658.562 kb on - strand, within BPHYT_RS22735
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 655,851 - BPHYT_RS22725 0.71 -0.2 655,858 - BPHYT_RS22725 0.72 -0.9 655,904 + BPHYT_RS22725 0.73 +0.9 656,601 + +0.9 656,711 + +0.2 656,846 + +2.5 656,847 - +0.1 657,527 - BPHYT_RS22730 0.60 -0.7 657,734 + BPHYT_RS22730 0.77 -2.1 658,562 - BPHYT_RS22735 0.65 +0.1 658,562 - BPHYT_RS22735 0.65 -1.9
Or see this region's nucleotide sequence