Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20235 and BPHYT_RS20240 overlap by 4 nucleotides BPHYT_RS20240 and BPHYT_RS20245 are separated by 5 nucleotides BPHYT_RS20245 and BPHYT_RS20250 are separated by 92 nucleotides BPHYT_RS20250 and BPHYT_RS20255 are separated by 101 nucleotides
BPHYT_RS20235: BPHYT_RS20235 - iron ABC transporter, at 83,827 to 85,935
_RS20235
BPHYT_RS20240: BPHYT_RS20240 - iron reductase, at 85,932 to 86,750
_RS20240
BPHYT_RS20245: BPHYT_RS20245 - iron ABC transporter substrate-binding protein, at 86,756 to 87,856
_RS20245
BPHYT_RS20250: BPHYT_RS20250 - dehydrogenase, at 87,949 to 88,134
_RS20250
BPHYT_RS20255: BPHYT_RS20255 - peptide ABC transporter ATP-binding protein, at 88,236 to 90,002
_RS20255
Position (kb) 86
87
88 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 86.202 kb on + strand, within BPHYT_RS20240 at 86.203 kb on - strand, within BPHYT_RS20240 at 86.205 kb on + strand, within BPHYT_RS20240 at 86.205 kb on + strand, within BPHYT_RS20240 at 86.206 kb on - strand, within BPHYT_RS20240 at 86.478 kb on + strand, within BPHYT_RS20240 at 86.650 kb on + strand, within BPHYT_RS20240 at 86.651 kb on - strand, within BPHYT_RS20240 at 86.651 kb on - strand, within BPHYT_RS20240 at 86.651 kb on - strand, within BPHYT_RS20240 at 86.655 kb on + strand, within BPHYT_RS20240 at 86.702 kb on - strand at 86.867 kb on + strand, within BPHYT_RS20245 at 87.089 kb on + strand, within BPHYT_RS20245 at 87.092 kb on + strand, within BPHYT_RS20245 at 87.275 kb on + strand, within BPHYT_RS20245 at 87.276 kb on - strand, within BPHYT_RS20245 at 87.593 kb on + strand, within BPHYT_RS20245 at 87.796 kb on + strand at 87.808 kb on + strand at 87.808 kb on + strand at 87.808 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 86,202 + BPHYT_RS20240 0.33 -1.0 86,203 - BPHYT_RS20240 0.33 -1.5 86,205 + BPHYT_RS20240 0.33 +0.1 86,205 + BPHYT_RS20240 0.33 -1.3 86,206 - BPHYT_RS20240 0.33 -3.0 86,478 + BPHYT_RS20240 0.67 +1.6 86,650 + BPHYT_RS20240 0.88 +0.8 86,651 - BPHYT_RS20240 0.88 -0.3 86,651 - BPHYT_RS20240 0.88 +0.0 86,651 - BPHYT_RS20240 0.88 +1.3 86,655 + BPHYT_RS20240 0.88 -0.5 86,702 - -2.4 86,867 + BPHYT_RS20245 0.10 +1.6 87,089 + BPHYT_RS20245 0.30 -1.8 87,092 + BPHYT_RS20245 0.31 -0.2 87,275 + BPHYT_RS20245 0.47 -1.4 87,276 - BPHYT_RS20245 0.47 -2.9 87,593 + BPHYT_RS20245 0.76 -3.5 87,796 + -0.0 87,808 + +0.3 87,808 + -2.2 87,808 + -0.3
Or see this region's nucleotide sequence