Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18570 and BPHYT_RS18575 are separated by 3 nucleotides BPHYT_RS18575 and BPHYT_RS18580 are separated by 90 nucleotides BPHYT_RS18580 and BPHYT_RS18585 are separated by 13 nucleotides
BPHYT_RS18570: BPHYT_RS18570 - tRNA 2-thiocytidine biosynthesis protein TtcA, at 4,178,299 to 4,179,309
_RS18570
BPHYT_RS18575: BPHYT_RS18575 - diguanylate cyclase, at 4,179,313 to 4,179,708
_RS18575
BPHYT_RS18580: BPHYT_RS18580 - short-chain dehydrogenase, at 4,179,799 to 4,180,617
_RS18580
BPHYT_RS18585: BPHYT_RS18585 - hypothetical protein, at 4,180,631 to 4,181,821
_RS18585
Position (kb) 4179
4180
4181 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4178.904 kb on - strand, within BPHYT_RS18570 at 4178.904 kb on - strand, within BPHYT_RS18570 at 4179.068 kb on + strand, within BPHYT_RS18570 at 4179.068 kb on + strand, within BPHYT_RS18570 at 4179.069 kb on - strand, within BPHYT_RS18570 at 4179.069 kb on - strand, within BPHYT_RS18570 at 4179.188 kb on + strand, within BPHYT_RS18570 at 4179.189 kb on - strand, within BPHYT_RS18570 at 4179.189 kb on - strand, within BPHYT_RS18570 at 4179.922 kb on - strand, within BPHYT_RS18580 at 4179.955 kb on - strand, within BPHYT_RS18580 at 4179.955 kb on - strand, within BPHYT_RS18580 at 4179.958 kb on - strand, within BPHYT_RS18580 at 4179.958 kb on - strand, within BPHYT_RS18580 at 4180.104 kb on - strand, within BPHYT_RS18580 at 4180.112 kb on + strand, within BPHYT_RS18580 at 4180.130 kb on + strand, within BPHYT_RS18580 at 4180.131 kb on - strand, within BPHYT_RS18580 at 4180.131 kb on - strand, within BPHYT_RS18580 at 4180.275 kb on - strand, within BPHYT_RS18580 at 4180.479 kb on - strand, within BPHYT_RS18580 at 4180.620 kb on - strand at 4180.663 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 4,178,904 - BPHYT_RS18570 0.60 +0.5 4,178,904 - BPHYT_RS18570 0.60 -2.7 4,179,068 + BPHYT_RS18570 0.76 -1.3 4,179,068 + BPHYT_RS18570 0.76 +0.3 4,179,069 - BPHYT_RS18570 0.76 -1.2 4,179,069 - BPHYT_RS18570 0.76 -0.3 4,179,188 + BPHYT_RS18570 0.88 -0.9 4,179,189 - BPHYT_RS18570 0.88 +0.2 4,179,189 - BPHYT_RS18570 0.88 -0.0 4,179,922 - BPHYT_RS18580 0.15 +0.0 4,179,955 - BPHYT_RS18580 0.19 -3.0 4,179,955 - BPHYT_RS18580 0.19 -2.6 4,179,958 - BPHYT_RS18580 0.19 -0.8 4,179,958 - BPHYT_RS18580 0.19 -0.3 4,180,104 - BPHYT_RS18580 0.37 -1.1 4,180,112 + BPHYT_RS18580 0.38 +1.5 4,180,130 + BPHYT_RS18580 0.40 -0.3 4,180,131 - BPHYT_RS18580 0.41 -1.1 4,180,131 - BPHYT_RS18580 0.41 -1.0 4,180,275 - BPHYT_RS18580 0.58 -0.5 4,180,479 - BPHYT_RS18580 0.83 +0.0 4,180,620 - +0.4 4,180,663 - -1.6
Or see this region's nucleotide sequence