Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16015 and BPHYT_RS16020 are separated by 18 nucleotides BPHYT_RS16020 and BPHYT_RS16025 are separated by 130 nucleotides BPHYT_RS16025 and BPHYT_RS16030 are separated by 28 nucleotides
BPHYT_RS16015: BPHYT_RS16015 - flavin reductase, at 3,634,169 to 3,634,678
_RS16015
BPHYT_RS16020: BPHYT_RS16020 - AsnC family transcriptional regulator, at 3,634,697 to 3,635,212
_RS16020
BPHYT_RS16025: BPHYT_RS16025 - Kynurenine formamidase, bacterial (EC 3.5.1.9) (from data) , at 3,635,343 to 3,635,981
_RS16025
BPHYT_RS16030: BPHYT_RS16030 - Kynureninase (EC 3.7.1.3) (from data) , at 3,636,010 to 3,637,260
_RS16030
Position (kb)
3635
3636 Strain fitness (log2 ratio)
-2
-1
0
1 at 3634.391 kb on + strand, within BPHYT_RS16015 at 3634.392 kb on - strand, within BPHYT_RS16015 at 3634.541 kb on - strand, within BPHYT_RS16015 at 3634.888 kb on - strand, within BPHYT_RS16020 at 3635.035 kb on + strand, within BPHYT_RS16020 at 3635.049 kb on + strand, within BPHYT_RS16020 at 3635.343 kb on - strand at 3635.517 kb on + strand, within BPHYT_RS16025 at 3635.517 kb on + strand, within BPHYT_RS16025 at 3635.518 kb on - strand, within BPHYT_RS16025 at 3635.663 kb on - strand, within BPHYT_RS16025 at 3635.673 kb on + strand, within BPHYT_RS16025 at 3636.112 kb on - strand at 3636.410 kb on - strand, within BPHYT_RS16030 at 3636.449 kb on - strand, within BPHYT_RS16030 at 3636.686 kb on - strand, within BPHYT_RS16030 at 3636.687 kb on + strand, within BPHYT_RS16030 at 3636.687 kb on + strand, within BPHYT_RS16030 at 3636.688 kb on - strand, within BPHYT_RS16030 at 3636.688 kb on - strand, within BPHYT_RS16030 at 3636.692 kb on - strand, within BPHYT_RS16030 at 3636.735 kb on + strand, within BPHYT_RS16030 at 3636.735 kb on + strand, within BPHYT_RS16030
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 3,634,391 + BPHYT_RS16015 0.44 -2.1 3,634,392 - BPHYT_RS16015 0.44 -2.6 3,634,541 - BPHYT_RS16015 0.73 +0.9 3,634,888 - BPHYT_RS16020 0.37 -0.3 3,635,035 + BPHYT_RS16020 0.66 +1.5 3,635,049 + BPHYT_RS16020 0.68 -0.1 3,635,343 - -0.4 3,635,517 + BPHYT_RS16025 0.27 -1.8 3,635,517 + BPHYT_RS16025 0.27 -2.0 3,635,518 - BPHYT_RS16025 0.27 -0.3 3,635,663 - BPHYT_RS16025 0.50 -0.4 3,635,673 + BPHYT_RS16025 0.52 -0.6 3,636,112 - -0.5 3,636,410 - BPHYT_RS16030 0.32 -1.5 3,636,449 - BPHYT_RS16030 0.35 -1.3 3,636,686 - BPHYT_RS16030 0.54 -0.1 3,636,687 + BPHYT_RS16030 0.54 -1.4 3,636,687 + BPHYT_RS16030 0.54 -1.0 3,636,688 - BPHYT_RS16030 0.54 +0.9 3,636,688 - BPHYT_RS16030 0.54 -0.4 3,636,692 - BPHYT_RS16030 0.55 -1.1 3,636,735 + BPHYT_RS16030 0.58 -1.8 3,636,735 + BPHYT_RS16030 0.58 +1.6
Or see this region's nucleotide sequence