Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS15170

Experiment: 36hr PHYTO6 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS15165 and BPHYT_RS15170 overlap by 14 nucleotidesBPHYT_RS15170 and BPHYT_RS15175 overlap by 4 nucleotides BPHYT_RS15165: BPHYT_RS15165 - histidine ammonia-lyase, at 3,450,204 to 3,451,799 _RS15165 BPHYT_RS15170: BPHYT_RS15170 - family 2 glycosyl transferase, at 3,451,786 to 3,453,513 _RS15170 BPHYT_RS15175: BPHYT_RS15175 - AMP-dependent synthetase, at 3,453,510 to 3,455,216 _RS15175 Position (kb) 3451 3452 3453 3454Strain fitness (log2 ratio) -1 0 1 2at 3451.288 kb on + strand, within BPHYT_RS15165at 3451.564 kb on + strand, within BPHYT_RS15165at 3452.042 kb on + strand, within BPHYT_RS15170at 3452.490 kb on + strand, within BPHYT_RS15170at 3452.491 kb on - strand, within BPHYT_RS15170at 3452.564 kb on + strand, within BPHYT_RS15170at 3452.832 kb on + strand, within BPHYT_RS15170at 3452.898 kb on - strand, within BPHYT_RS15170at 3452.898 kb on - strand, within BPHYT_RS15170at 3452.943 kb on - strand, within BPHYT_RS15170at 3453.805 kb on + strand, within BPHYT_RS15175at 3454.117 kb on + strand, within BPHYT_RS15175at 3454.117 kb on + strand, within BPHYT_RS15175

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10
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3,451,288 + BPHYT_RS15165 0.68 +0.4
3,451,564 + BPHYT_RS15165 0.85 -0.5
3,452,042 + BPHYT_RS15170 0.15 +2.0
3,452,490 + BPHYT_RS15170 0.41 +1.0
3,452,491 - BPHYT_RS15170 0.41 +2.4
3,452,564 + BPHYT_RS15170 0.45 +0.6
3,452,832 + BPHYT_RS15170 0.61 -0.2
3,452,898 - BPHYT_RS15170 0.64 -0.7
3,452,898 - BPHYT_RS15170 0.64 +0.3
3,452,943 - BPHYT_RS15170 0.67 +0.9
3,453,805 + BPHYT_RS15175 0.17 -0.3
3,454,117 + BPHYT_RS15175 0.36 +0.7
3,454,117 + BPHYT_RS15175 0.36 +1.7

Or see this region's nucleotide sequence