Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14005 and BPHYT_RS14010 are separated by 26 nucleotides BPHYT_RS14010 and BPHYT_RS14015 are separated by 106 nucleotides BPHYT_RS14015 and BPHYT_RS14020 are separated by 286 nucleotides BPHYT_RS14020 and BPHYT_RS14025 are separated by 48 nucleotides
BPHYT_RS14005: BPHYT_RS14005 - ABC transporter, at 3,201,887 to 3,202,765
_RS14005
BPHYT_RS14010: BPHYT_RS14010 - NUDIX hydrolase, at 3,202,792 to 3,203,241
_RS14010
BPHYT_RS14015: BPHYT_RS14015 - hypothetical protein, at 3,203,348 to 3,203,737
_RS14015
BPHYT_RS14020: BPHYT_RS14020 - hypothetical protein, at 3,204,024 to 3,204,263
_RS14020
BPHYT_RS14025: BPHYT_RS14025 - cytochrome C peroxidase, at 3,204,312 to 3,205,694
_RS14025
Position (kb)
3203
3204 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3202.573 kb on + strand, within BPHYT_RS14005 at 3202.653 kb on - strand, within BPHYT_RS14005 at 3202.862 kb on + strand, within BPHYT_RS14010 at 3202.862 kb on + strand, within BPHYT_RS14010 at 3202.862 kb on + strand, within BPHYT_RS14010 at 3202.863 kb on - strand, within BPHYT_RS14010 at 3202.863 kb on - strand, within BPHYT_RS14010 at 3203.027 kb on + strand, within BPHYT_RS14010 at 3203.226 kb on + strand at 3203.515 kb on + strand, within BPHYT_RS14015 at 3203.515 kb on + strand, within BPHYT_RS14015 at 3203.622 kb on + strand, within BPHYT_RS14015 at 3203.622 kb on + strand, within BPHYT_RS14015 at 3203.622 kb on + strand, within BPHYT_RS14015 at 3203.811 kb on - strand at 3203.915 kb on + strand at 3203.916 kb on - strand at 3203.935 kb on - strand at 3204.027 kb on + strand at 3204.028 kb on - strand at 3204.028 kb on - strand at 3204.028 kb on - strand at 3204.028 kb on - strand at 3204.028 kb on - strand at 3204.234 kb on + strand, within BPHYT_RS14020 at 3204.234 kb on + strand, within BPHYT_RS14020 at 3204.234 kb on + strand, within BPHYT_RS14020 at 3204.235 kb on - strand, within BPHYT_RS14020 at 3204.237 kb on + strand, within BPHYT_RS14020 at 3204.237 kb on + strand, within BPHYT_RS14020 at 3204.237 kb on + strand, within BPHYT_RS14020 at 3204.238 kb on - strand, within BPHYT_RS14020 at 3204.238 kb on - strand, within BPHYT_RS14020 at 3204.238 kb on - strand at 3204.238 kb on - strand, within BPHYT_RS14020 at 3204.355 kb on + strand at 3204.356 kb on - strand at 3204.356 kb on - strand at 3204.371 kb on + strand at 3204.472 kb on + strand, within BPHYT_RS14025 at 3204.557 kb on - strand, within BPHYT_RS14025 at 3204.631 kb on - strand, within BPHYT_RS14025 at 3204.712 kb on + strand, within BPHYT_RS14025
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 3,202,573 + BPHYT_RS14005 0.78 -0.1 3,202,653 - BPHYT_RS14005 0.87 -0.6 3,202,862 + BPHYT_RS14010 0.16 -1.4 3,202,862 + BPHYT_RS14010 0.16 -1.4 3,202,862 + BPHYT_RS14010 0.16 +0.7 3,202,863 - BPHYT_RS14010 0.16 +1.0 3,202,863 - BPHYT_RS14010 0.16 -0.9 3,203,027 + BPHYT_RS14010 0.52 +1.5 3,203,226 + +0.5 3,203,515 + BPHYT_RS14015 0.43 -2.2 3,203,515 + BPHYT_RS14015 0.43 -1.0 3,203,622 + BPHYT_RS14015 0.70 -0.7 3,203,622 + BPHYT_RS14015 0.70 -1.8 3,203,622 + BPHYT_RS14015 0.70 +0.0 3,203,811 - +0.1 3,203,915 + -0.2 3,203,916 - +0.9 3,203,935 - -1.8 3,204,027 + -0.2 3,204,028 - -0.3 3,204,028 - -0.2 3,204,028 - -0.8 3,204,028 - -0.5 3,204,028 - +2.5 3,204,234 + BPHYT_RS14020 0.88 +1.0 3,204,234 + BPHYT_RS14020 0.88 -1.1 3,204,234 + BPHYT_RS14020 0.88 +0.1 3,204,235 - BPHYT_RS14020 0.88 -0.1 3,204,237 + BPHYT_RS14020 0.89 +0.9 3,204,237 + BPHYT_RS14020 0.89 -0.4 3,204,237 + BPHYT_RS14020 0.89 +0.4 3,204,238 - BPHYT_RS14020 0.89 +0.3 3,204,238 - BPHYT_RS14020 0.89 -2.3 3,204,238 - +0.9 3,204,238 - BPHYT_RS14020 0.89 -2.1 3,204,355 + -0.1 3,204,356 - -0.3 3,204,356 - -1.0 3,204,371 + +0.1 3,204,472 + BPHYT_RS14025 0.12 +0.1 3,204,557 - BPHYT_RS14025 0.18 -0.9 3,204,631 - BPHYT_RS14025 0.23 +0.5 3,204,712 + BPHYT_RS14025 0.29 -0.5
Or see this region's nucleotide sequence