Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11110

Experiment: 36hr PHYTO6 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11105 and BPHYT_RS11110 are separated by 197 nucleotidesBPHYT_RS11110 and BPHYT_RS11115 are separated by 191 nucleotidesBPHYT_RS11115 and BPHYT_RS11120 are separated by 82 nucleotides BPHYT_RS11105: BPHYT_RS11105 - acyl-CoA dehydrogenase, at 2,523,066 to 2,524,244 _RS11105 BPHYT_RS11110: BPHYT_RS11110 - chemotaxis protein CheY, at 2,524,442 to 2,526,415 _RS11110 BPHYT_RS11115: BPHYT_RS11115 - hypothetical protein, at 2,526,607 to 2,526,849 _RS11115 BPHYT_RS11120: BPHYT_RS11120 - ferredoxin--NADP reductase, at 2,526,932 to 2,527,981 _RS11120 Position (kb) 2524 2525 2526 2527Strain fitness (log2 ratio) -1 0 1 2at 2523.540 kb on + strand, within BPHYT_RS11105at 2523.544 kb on + strand, within BPHYT_RS11105at 2524.647 kb on + strand, within BPHYT_RS11110at 2524.907 kb on - strand, within BPHYT_RS11110at 2525.252 kb on - strand, within BPHYT_RS11110at 2525.252 kb on - strand, within BPHYT_RS11110at 2525.794 kb on - strand, within BPHYT_RS11110at 2525.878 kb on - strand, within BPHYT_RS11110at 2526.177 kb on + strand, within BPHYT_RS11110at 2526.321 kb on + strandat 2526.429 kb on - strandat 2527.015 kb on - strandat 2527.020 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10
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2,523,540 + BPHYT_RS11105 0.40 -0.8
2,523,544 + BPHYT_RS11105 0.41 -0.2
2,524,647 + BPHYT_RS11110 0.10 -0.7
2,524,907 - BPHYT_RS11110 0.24 -1.1
2,525,252 - BPHYT_RS11110 0.41 -1.4
2,525,252 - BPHYT_RS11110 0.41 +0.5
2,525,794 - BPHYT_RS11110 0.68 -0.8
2,525,878 - BPHYT_RS11110 0.73 +0.5
2,526,177 + BPHYT_RS11110 0.88 -1.3
2,526,321 + -0.8
2,526,429 - +1.0
2,527,015 - -1.1
2,527,020 + +1.9

Or see this region's nucleotide sequence