Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07410 and BPHYT_RS07415 are separated by 391 nucleotides BPHYT_RS07415 and BPHYT_RS07420 overlap by 1 nucleotides BPHYT_RS07420 and BPHYT_RS07425 are separated by 294 nucleotides
BPHYT_RS07410: BPHYT_RS07410 - glucosamine--fructose-6-phosphate aminotransferase, at 1,684,887 to 1,686,704
_RS07410
BPHYT_RS07415: BPHYT_RS07415 - hypothetical protein, at 1,687,096 to 1,687,419
_RS07415
BPHYT_RS07420: BPHYT_RS07420 - hypothetical protein, at 1,687,419 to 1,687,637
_RS07420
BPHYT_RS07425: BPHYT_RS07425 - hypothetical protein, at 1,687,932 to 1,688,723
_RS07425
Position (kb) 1687
1688 Strain fitness (log2 ratio) -2
-1
0
1 at 1686.349 kb on - strand at 1686.507 kb on + strand, within BPHYT_RS07410 at 1686.508 kb on - strand, within BPHYT_RS07410 at 1687.059 kb on - strand at 1687.138 kb on + strand, within BPHYT_RS07415 at 1687.138 kb on + strand, within BPHYT_RS07415 at 1687.138 kb on + strand, within BPHYT_RS07415 at 1687.139 kb on - strand, within BPHYT_RS07415 at 1687.435 kb on - strand at 1687.572 kb on - strand, within BPHYT_RS07420 at 1688.010 kb on - strand at 1688.099 kb on - strand, within BPHYT_RS07425 at 1688.360 kb on - strand, within BPHYT_RS07425
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 1,686,349 - +0.9 1,686,507 + BPHYT_RS07410 0.89 +0.3 1,686,508 - BPHYT_RS07410 0.89 -1.5 1,687,059 - -2.0 1,687,138 + BPHYT_RS07415 0.13 +0.1 1,687,138 + BPHYT_RS07415 0.13 +1.0 1,687,138 + BPHYT_RS07415 0.13 +1.3 1,687,139 - BPHYT_RS07415 0.13 +0.5 1,687,435 - -1.5 1,687,572 - BPHYT_RS07420 0.70 -0.3 1,688,010 - +0.0 1,688,099 - BPHYT_RS07425 0.21 +1.0 1,688,360 - BPHYT_RS07425 0.54 +0.4
Or see this region's nucleotide sequence