Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05145 and BPHYT_RS05150 are separated by 401 nucleotides BPHYT_RS05150 and BPHYT_RS05155 are separated by 199 nucleotides BPHYT_RS05155 and BPHYT_RS05160 are separated by 222 nucleotides
BPHYT_RS05145: BPHYT_RS05145 - hypothetical protein, at 1,186,779 to 1,187,759
_RS05145
BPHYT_RS05150: BPHYT_RS05150 - methionine aminopeptidase, at 1,188,161 to 1,188,946
_RS05150
BPHYT_RS05155: BPHYT_RS05155 - acetyltransferase GCN5, at 1,189,146 to 1,189,679
_RS05155
BPHYT_RS05160: BPHYT_RS05160 - septum site-determining protein MinC, at 1,189,902 to 1,190,756
_RS05160
Position (kb)
1188
1189 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1187.185 kb on + strand, within BPHYT_RS05145 at 1187.185 kb on + strand, within BPHYT_RS05145 at 1187.507 kb on - strand, within BPHYT_RS05145 at 1187.511 kb on - strand, within BPHYT_RS05145 at 1187.530 kb on + strand, within BPHYT_RS05145 at 1188.169 kb on + strand at 1188.169 kb on + strand at 1188.170 kb on - strand at 1188.173 kb on + strand at 1188.174 kb on - strand at 1188.303 kb on - strand, within BPHYT_RS05150 at 1188.304 kb on + strand, within BPHYT_RS05150 at 1188.304 kb on + strand, within BPHYT_RS05150 at 1188.304 kb on + strand, within BPHYT_RS05150 at 1188.305 kb on - strand, within BPHYT_RS05150 at 1188.305 kb on - strand, within BPHYT_RS05150 at 1188.305 kb on - strand, within BPHYT_RS05150 at 1188.497 kb on + strand, within BPHYT_RS05150 at 1188.498 kb on - strand, within BPHYT_RS05150 at 1188.548 kb on + strand, within BPHYT_RS05150 at 1188.549 kb on - strand, within BPHYT_RS05150 at 1188.549 kb on - strand, within BPHYT_RS05150 at 1188.549 kb on - strand, within BPHYT_RS05150 at 1188.549 kb on - strand at 1188.690 kb on - strand, within BPHYT_RS05150 at 1188.716 kb on + strand, within BPHYT_RS05150 at 1188.894 kb on - strand at 1189.114 kb on - strand at 1189.114 kb on - strand at 1189.212 kb on - strand, within BPHYT_RS05155 at 1189.236 kb on + strand, within BPHYT_RS05155 at 1189.237 kb on - strand, within BPHYT_RS05155 at 1189.518 kb on + strand, within BPHYT_RS05155 at 1189.518 kb on + strand, within BPHYT_RS05155 at 1189.560 kb on + strand, within BPHYT_RS05155 at 1189.561 kb on - strand, within BPHYT_RS05155 at 1189.901 kb on + strand at 1189.902 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 1,187,185 + BPHYT_RS05145 0.41 +0.9 1,187,185 + BPHYT_RS05145 0.41 -0.3 1,187,507 - BPHYT_RS05145 0.74 -1.2 1,187,511 - BPHYT_RS05145 0.75 +0.3 1,187,530 + BPHYT_RS05145 0.77 +0.2 1,188,169 + +0.7 1,188,169 + -2.6 1,188,170 - -1.9 1,188,173 + -1.9 1,188,174 - -0.3 1,188,303 - BPHYT_RS05150 0.18 +0.4 1,188,304 + BPHYT_RS05150 0.18 -0.5 1,188,304 + BPHYT_RS05150 0.18 -1.3 1,188,304 + BPHYT_RS05150 0.18 -0.8 1,188,305 - BPHYT_RS05150 0.18 +1.2 1,188,305 - BPHYT_RS05150 0.18 +0.4 1,188,305 - BPHYT_RS05150 0.18 -0.4 1,188,497 + BPHYT_RS05150 0.43 +0.8 1,188,498 - BPHYT_RS05150 0.43 -0.1 1,188,548 + BPHYT_RS05150 0.49 -1.4 1,188,549 - BPHYT_RS05150 0.49 -0.5 1,188,549 - BPHYT_RS05150 0.49 -0.1 1,188,549 - BPHYT_RS05150 0.49 -1.1 1,188,549 - -1.1 1,188,690 - BPHYT_RS05150 0.67 -0.2 1,188,716 + BPHYT_RS05150 0.71 -1.6 1,188,894 - -0.6 1,189,114 - +1.5 1,189,114 - +2.0 1,189,212 - BPHYT_RS05155 0.12 +2.1 1,189,236 + BPHYT_RS05155 0.17 +1.0 1,189,237 - BPHYT_RS05155 0.17 +1.9 1,189,518 + BPHYT_RS05155 0.70 +1.3 1,189,518 + BPHYT_RS05155 0.70 +1.8 1,189,560 + BPHYT_RS05155 0.78 +1.6 1,189,561 - BPHYT_RS05155 0.78 +2.1 1,189,901 + -1.7 1,189,902 - -0.7
Or see this region's nucleotide sequence