Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01335 and BPHYT_RS01340 are separated by 428 nucleotides BPHYT_RS01340 and BPHYT_RS01345 are separated by 17 nucleotides BPHYT_RS01345 and BPHYT_RS01350 are separated by 107 nucleotides
BPHYT_RS01335: BPHYT_RS01335 - hypothetical protein, at 306,213 to 306,437
_RS01335
BPHYT_RS01340: BPHYT_RS01340 - ChrB domain-containing protein, at 306,866 to 307,429
_RS01340
BPHYT_RS01345: BPHYT_RS01345 - endonuclease/exonuclease/phosphatase, at 307,447 to 308,253
_RS01345
BPHYT_RS01350: BPHYT_RS01350 - MFS transporter permease, at 308,361 to 309,623
_RS01350
Position (kb) 306
307
308 Strain fitness (log2 ratio) -1
0
1
2 at 305.924 kb on + strand at 305.924 kb on + strand at 305.925 kb on - strand at 305.925 kb on - strand at 305.925 kb on - strand at 305.925 kb on - strand at 306.062 kb on + strand at 306.199 kb on + strand at 306.200 kb on - strand at 306.227 kb on + strand at 306.228 kb on - strand at 306.466 kb on + strand at 306.467 kb on - strand at 306.468 kb on + strand at 306.469 kb on - strand at 306.480 kb on + strand at 306.712 kb on + strand at 306.713 kb on - strand at 306.897 kb on - strand at 306.968 kb on + strand, within BPHYT_RS01340 at 307.223 kb on + strand, within BPHYT_RS01340 at 307.223 kb on + strand, within BPHYT_RS01340 at 307.367 kb on + strand, within BPHYT_RS01340 at 307.367 kb on + strand, within BPHYT_RS01340 at 307.427 kb on + strand at 307.427 kb on + strand at 307.467 kb on + strand at 307.468 kb on - strand at 307.471 kb on + strand at 307.471 kb on + strand at 307.471 kb on + strand at 307.472 kb on - strand at 307.472 kb on - strand at 307.472 kb on - strand at 307.978 kb on + strand, within BPHYT_RS01345 at 307.978 kb on + strand, within BPHYT_RS01345 at 307.979 kb on - strand, within BPHYT_RS01345 at 308.137 kb on + strand, within BPHYT_RS01345 at 308.225 kb on - strand at 308.251 kb on + strand at 308.252 kb on - strand at 308.252 kb on - strand at 308.296 kb on + strand at 308.296 kb on + strand at 308.296 kb on + strand at 308.297 kb on - strand at 308.301 kb on + strand at 308.301 kb on + strand at 308.301 kb on + strand at 308.301 kb on + strand at 308.302 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 305,924 + -0.6 305,924 + +1.0 305,925 - -0.2 305,925 - +0.2 305,925 - -0.6 305,925 - -1.3 306,062 + -0.5 306,199 + -1.4 306,200 - -0.3 306,227 + +0.9 306,228 - +0.1 306,466 + +0.6 306,467 - -1.0 306,468 + +0.6 306,469 - +0.3 306,480 + +0.3 306,712 + -1.2 306,713 - -0.1 306,897 - +0.3 306,968 + BPHYT_RS01340 0.18 +0.6 307,223 + BPHYT_RS01340 0.63 -1.0 307,223 + BPHYT_RS01340 0.63 +1.6 307,367 + BPHYT_RS01340 0.89 -0.6 307,367 + BPHYT_RS01340 0.89 -0.6 307,427 + +0.6 307,427 + -1.0 307,467 + -0.2 307,468 - -0.9 307,471 + -1.7 307,471 + +0.0 307,471 + +0.4 307,472 - +0.8 307,472 - +0.0 307,472 - -0.0 307,978 + BPHYT_RS01345 0.66 +0.8 307,978 + BPHYT_RS01345 0.66 +2.4 307,979 - BPHYT_RS01345 0.66 +0.2 308,137 + BPHYT_RS01345 0.86 -0.1 308,225 - -1.1 308,251 + -0.2 308,252 - +0.0 308,252 - +0.3 308,296 + +2.6 308,296 + -0.9 308,296 + +0.3 308,297 - -1.3 308,301 + +0.7 308,301 + -0.9 308,301 + -0.7 308,301 + +2.2 308,302 - -1.0
Or see this region's nucleotide sequence