Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00675 and BPHYT_RS00680 are separated by 54 nucleotides BPHYT_RS00680 and BPHYT_RS00685 are separated by 330 nucleotides BPHYT_RS00685 and BPHYT_RS00690 are separated by 57 nucleotides
BPHYT_RS00675: BPHYT_RS00675 - GntR family transcriptional regulator, at 159,334 to 160,170
_RS00675
BPHYT_RS00680: BPHYT_RS00680 - amidase, at 160,225 to 161,649
_RS00680
BPHYT_RS00685: BPHYT_RS00685 - transcriptional regulator, at 161,980 to 162,309
_RS00685
BPHYT_RS00690: BPHYT_RS00690 - transcriptional regulator, at 162,367 to 162,726
_RS00690
Position (kb) 160
161
162 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 159.287 kb on + strand at 159.320 kb on + strand at 159.320 kb on + strand at 159.321 kb on - strand at 159.321 kb on - strand at 159.333 kb on + strand at 159.333 kb on + strand at 159.333 kb on + strand at 159.334 kb on - strand at 159.606 kb on + strand, within BPHYT_RS00675 at 159.607 kb on - strand, within BPHYT_RS00675 at 159.649 kb on + strand, within BPHYT_RS00675 at 159.650 kb on - strand, within BPHYT_RS00675 at 159.685 kb on + strand, within BPHYT_RS00675 at 159.685 kb on + strand, within BPHYT_RS00675 at 159.685 kb on + strand, within BPHYT_RS00675 at 159.686 kb on - strand, within BPHYT_RS00675 at 159.686 kb on - strand, within BPHYT_RS00675 at 159.714 kb on - strand, within BPHYT_RS00675 at 159.783 kb on - strand, within BPHYT_RS00675 at 159.921 kb on + strand, within BPHYT_RS00675 at 159.922 kb on - strand, within BPHYT_RS00675 at 159.961 kb on + strand, within BPHYT_RS00675 at 159.962 kb on - strand, within BPHYT_RS00675 at 159.962 kb on - strand, within BPHYT_RS00675 at 160.216 kb on + strand at 160.220 kb on + strand at 160.220 kb on + strand at 160.221 kb on - strand at 160.221 kb on - strand at 160.221 kb on - strand at 160.242 kb on + strand at 160.401 kb on - strand, within BPHYT_RS00680 at 160.405 kb on - strand, within BPHYT_RS00680 at 160.405 kb on - strand, within BPHYT_RS00680 at 160.405 kb on - strand, within BPHYT_RS00680 at 160.505 kb on + strand, within BPHYT_RS00680 at 160.506 kb on - strand, within BPHYT_RS00680 at 160.506 kb on - strand, within BPHYT_RS00680 at 160.506 kb on - strand, within BPHYT_RS00680 at 160.506 kb on - strand, within BPHYT_RS00680 at 160.628 kb on + strand, within BPHYT_RS00680 at 160.628 kb on + strand, within BPHYT_RS00680 at 160.629 kb on - strand, within BPHYT_RS00680 at 160.629 kb on - strand, within BPHYT_RS00680 at 160.681 kb on - strand, within BPHYT_RS00680 at 160.856 kb on + strand, within BPHYT_RS00680 at 160.856 kb on + strand, within BPHYT_RS00680 at 160.856 kb on + strand, within BPHYT_RS00680 at 160.857 kb on - strand, within BPHYT_RS00680 at 160.857 kb on - strand, within BPHYT_RS00680 at 160.857 kb on - strand, within BPHYT_RS00680 at 160.862 kb on - strand, within BPHYT_RS00680 at 160.907 kb on + strand, within BPHYT_RS00680 at 160.934 kb on + strand, within BPHYT_RS00680 at 161.013 kb on + strand, within BPHYT_RS00680 at 161.085 kb on + strand, within BPHYT_RS00680 at 161.151 kb on + strand, within BPHYT_RS00680 at 161.151 kb on + strand, within BPHYT_RS00680 at 161.209 kb on - strand, within BPHYT_RS00680 at 161.507 kb on - strand at 161.599 kb on - strand at 161.599 kb on - strand at 161.601 kb on - strand at 161.601 kb on - strand at 161.601 kb on - strand at 161.602 kb on + strand at 161.603 kb on - strand at 161.649 kb on + strand at 161.649 kb on + strand at 161.649 kb on + strand at 161.650 kb on - strand at 161.650 kb on - strand at 161.673 kb on + strand at 161.684 kb on + strand at 161.781 kb on - strand at 161.787 kb on - strand at 161.837 kb on + strand at 162.086 kb on + strand, within BPHYT_RS00685 at 162.140 kb on - strand, within BPHYT_RS00685 at 162.292 kb on - strand at 162.298 kb on - strand at 162.634 kb on - strand, within BPHYT_RS00690
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 159,287 + -0.2 159,320 + +0.8 159,320 + -0.4 159,321 - +0.2 159,321 - +0.3 159,333 + +0.1 159,333 + -0.6 159,333 + +0.2 159,334 - +0.3 159,606 + BPHYT_RS00675 0.32 -0.2 159,607 - BPHYT_RS00675 0.33 +0.2 159,649 + BPHYT_RS00675 0.38 -1.6 159,650 - BPHYT_RS00675 0.38 -0.6 159,685 + BPHYT_RS00675 0.42 -1.3 159,685 + BPHYT_RS00675 0.42 -0.4 159,685 + BPHYT_RS00675 0.42 -1.7 159,686 - BPHYT_RS00675 0.42 -0.3 159,686 - BPHYT_RS00675 0.42 -0.8 159,714 - BPHYT_RS00675 0.45 -0.5 159,783 - BPHYT_RS00675 0.54 +2.6 159,921 + BPHYT_RS00675 0.70 +1.0 159,922 - BPHYT_RS00675 0.70 -1.5 159,961 + BPHYT_RS00675 0.75 -0.4 159,962 - BPHYT_RS00675 0.75 -2.1 159,962 - BPHYT_RS00675 0.75 -0.5 160,216 + -0.7 160,220 + -1.4 160,220 + +0.0 160,221 - +1.3 160,221 - +0.4 160,221 - +0.0 160,242 + +0.8 160,401 - BPHYT_RS00680 0.12 -0.2 160,405 - BPHYT_RS00680 0.13 -1.2 160,405 - BPHYT_RS00680 0.13 -1.3 160,405 - BPHYT_RS00680 0.13 +0.1 160,505 + BPHYT_RS00680 0.20 -0.4 160,506 - BPHYT_RS00680 0.20 -0.1 160,506 - BPHYT_RS00680 0.20 -0.4 160,506 - BPHYT_RS00680 0.20 -0.1 160,506 - BPHYT_RS00680 0.20 -0.4 160,628 + BPHYT_RS00680 0.28 -0.1 160,628 + BPHYT_RS00680 0.28 -0.4 160,629 - BPHYT_RS00680 0.28 +1.3 160,629 - BPHYT_RS00680 0.28 +0.9 160,681 - BPHYT_RS00680 0.32 +0.0 160,856 + BPHYT_RS00680 0.44 -0.2 160,856 + BPHYT_RS00680 0.44 -0.7 160,856 + BPHYT_RS00680 0.44 -0.6 160,857 - BPHYT_RS00680 0.44 -0.1 160,857 - BPHYT_RS00680 0.44 +0.1 160,857 - BPHYT_RS00680 0.44 +1.0 160,862 - BPHYT_RS00680 0.45 +0.4 160,907 + BPHYT_RS00680 0.48 -0.4 160,934 + BPHYT_RS00680 0.50 +1.8 161,013 + BPHYT_RS00680 0.55 +3.4 161,085 + BPHYT_RS00680 0.60 +2.0 161,151 + BPHYT_RS00680 0.65 +0.7 161,151 + BPHYT_RS00680 0.65 +0.3 161,209 - BPHYT_RS00680 0.69 +2.3 161,507 - -0.3 161,599 - +0.1 161,599 - +1.2 161,601 - +1.4 161,601 - +0.1 161,601 - +0.2 161,602 + -0.3 161,603 - -0.4 161,649 + -0.7 161,649 + -0.3 161,649 + +0.5 161,650 - +1.0 161,650 - +0.2 161,673 + -0.1 161,684 + -1.3 161,781 - -0.0 161,787 - +1.8 161,837 + +0.2 162,086 + BPHYT_RS00685 0.32 -0.2 162,140 - BPHYT_RS00685 0.48 +0.1 162,292 - +0.2 162,298 - -0.3 162,634 - BPHYT_RS00690 0.74 -0.3
Or see this region's nucleotide sequence