Experiment: 36hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00660 and BPHYT_RS00665 overlap by 17 nucleotides BPHYT_RS00665 and BPHYT_RS00670 overlap by 11 nucleotides BPHYT_RS00670 and BPHYT_RS00675 are separated by 239 nucleotides
BPHYT_RS00660: BPHYT_RS00660 - 3-oxoacyl-ACP synthase, at 156,510 to 157,532
_RS00660
BPHYT_RS00665: BPHYT_RS00665 - phosphopantetheine-binding protein, at 157,516 to 157,794
_RS00665
BPHYT_RS00670: BPHYT_RS00670 - acyl-CoA dehydrogenase, at 157,784 to 159,094
_RS00670
BPHYT_RS00675: BPHYT_RS00675 - GntR family transcriptional regulator, at 159,334 to 160,170
_RS00675
Position (kb) 157
158
159
160 Strain fitness (log2 ratio) -2
-1
0
1
2 at 156.869 kb on + strand, within BPHYT_RS00660 at 157.152 kb on + strand, within BPHYT_RS00660 at 157.298 kb on - strand, within BPHYT_RS00660 at 157.384 kb on + strand, within BPHYT_RS00660 at 157.385 kb on - strand, within BPHYT_RS00660 at 157.437 kb on + strand at 157.474 kb on + strand at 157.820 kb on + strand at 157.820 kb on + strand at 157.821 kb on - strand at 157.821 kb on - strand at 158.090 kb on - strand, within BPHYT_RS00670 at 158.319 kb on + strand, within BPHYT_RS00670 at 158.320 kb on - strand, within BPHYT_RS00670 at 158.415 kb on + strand, within BPHYT_RS00670 at 158.488 kb on + strand, within BPHYT_RS00670 at 158.488 kb on + strand, within BPHYT_RS00670 at 158.489 kb on - strand, within BPHYT_RS00670 at 158.520 kb on + strand, within BPHYT_RS00670 at 158.642 kb on - strand at 158.642 kb on - strand, within BPHYT_RS00670 at 159.084 kb on + strand at 159.085 kb on - strand at 159.146 kb on + strand at 159.172 kb on + strand at 159.173 kb on - strand at 159.186 kb on - strand at 159.221 kb on + strand at 159.287 kb on + strand at 159.320 kb on + strand at 159.320 kb on + strand at 159.321 kb on - strand at 159.321 kb on - strand at 159.333 kb on + strand at 159.333 kb on + strand at 159.333 kb on + strand at 159.334 kb on - strand at 159.606 kb on + strand, within BPHYT_RS00675 at 159.607 kb on - strand, within BPHYT_RS00675 at 159.649 kb on + strand, within BPHYT_RS00675 at 159.650 kb on - strand, within BPHYT_RS00675 at 159.685 kb on + strand, within BPHYT_RS00675 at 159.685 kb on + strand, within BPHYT_RS00675 at 159.685 kb on + strand, within BPHYT_RS00675 at 159.686 kb on - strand, within BPHYT_RS00675 at 159.686 kb on - strand, within BPHYT_RS00675 at 159.714 kb on - strand, within BPHYT_RS00675 at 159.783 kb on - strand, within BPHYT_RS00675 at 159.921 kb on + strand, within BPHYT_RS00675 at 159.922 kb on - strand, within BPHYT_RS00675 at 159.961 kb on + strand, within BPHYT_RS00675 at 159.962 kb on - strand, within BPHYT_RS00675 at 159.962 kb on - strand, within BPHYT_RS00675
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 10 remove 156,869 + BPHYT_RS00660 0.35 -0.6 157,152 + BPHYT_RS00660 0.63 +0.5 157,298 - BPHYT_RS00660 0.77 -1.1 157,384 + BPHYT_RS00660 0.85 -1.8 157,385 - BPHYT_RS00660 0.86 +0.3 157,437 + -1.7 157,474 + -0.1 157,820 + +0.3 157,820 + -0.9 157,821 - -0.7 157,821 - -1.2 158,090 - BPHYT_RS00670 0.23 +0.4 158,319 + BPHYT_RS00670 0.41 +1.5 158,320 - BPHYT_RS00670 0.41 -0.6 158,415 + BPHYT_RS00670 0.48 +0.2 158,488 + BPHYT_RS00670 0.54 -0.5 158,488 + BPHYT_RS00670 0.54 +0.0 158,489 - BPHYT_RS00670 0.54 -1.0 158,520 + BPHYT_RS00670 0.56 +0.4 158,642 - +2.6 158,642 - BPHYT_RS00670 0.65 +0.1 159,084 + +0.1 159,085 - -1.0 159,146 + +0.1 159,172 + -0.4 159,173 - +0.4 159,186 - -0.3 159,221 + +0.2 159,287 + -0.2 159,320 + +0.8 159,320 + -0.4 159,321 - +0.2 159,321 - +0.3 159,333 + +0.1 159,333 + -0.6 159,333 + +0.2 159,334 - +0.3 159,606 + BPHYT_RS00675 0.32 -0.2 159,607 - BPHYT_RS00675 0.33 +0.2 159,649 + BPHYT_RS00675 0.38 -1.6 159,650 - BPHYT_RS00675 0.38 -0.6 159,685 + BPHYT_RS00675 0.42 -1.3 159,685 + BPHYT_RS00675 0.42 -0.4 159,685 + BPHYT_RS00675 0.42 -1.7 159,686 - BPHYT_RS00675 0.42 -0.3 159,686 - BPHYT_RS00675 0.42 -0.8 159,714 - BPHYT_RS00675 0.45 -0.5 159,783 - BPHYT_RS00675 0.54 +2.6 159,921 + BPHYT_RS00675 0.70 +1.0 159,922 - BPHYT_RS00675 0.70 -1.5 159,961 + BPHYT_RS00675 0.75 -0.4 159,962 - BPHYT_RS00675 0.75 -2.1 159,962 - BPHYT_RS00675 0.75 -0.5
Or see this region's nucleotide sequence