Experiment: 36hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS36205 and BPHYT_RS36210 overlap by 4 nucleotides BPHYT_RS36210 and BPHYT_RS36215 are separated by 127 nucleotides BPHYT_RS36215 and BPHYT_RS36220 are separated by 51 nucleotides BPHYT_RS36220 and BPHYT_RS36225 overlap by 23 nucleotides BPHYT_RS36225 and BPHYT_RS36230 are separated by 55 nucleotides
BPHYT_RS36205: BPHYT_RS36205 - integrase, at 56,148 to 58,031
_RS36205
BPHYT_RS36210: BPHYT_RS36210 - hypothetical protein, at 58,028 to 58,390
_RS36210
BPHYT_RS36215: BPHYT_RS36215 - hypothetical protein, at 58,518 to 59,036
_RS36215
BPHYT_RS36220: BPHYT_RS36220 - hypothetical protein, at 59,088 to 59,288
_RS36220
BPHYT_RS36225: BPHYT_RS36225 - hypothetical protein, at 59,266 to 59,538
_RS36225
BPHYT_RS36230: BPHYT_RS36230 - hypothetical protein, at 59,594 to 59,788
_RS36230
Position (kb) 58
59
60 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 58.566 kb on - strand at 58.586 kb on - strand, within BPHYT_RS36215 at 58.774 kb on - strand, within BPHYT_RS36215 at 59.198 kb on + strand, within BPHYT_RS36220 at 59.329 kb on + strand, within BPHYT_RS36225 at 59.330 kb on - strand, within BPHYT_RS36225 at 59.362 kb on - strand, within BPHYT_RS36225 at 59.369 kb on - strand, within BPHYT_RS36225 at 59.392 kb on + strand, within BPHYT_RS36225 at 59.393 kb on - strand, within BPHYT_RS36225 at 59.393 kb on - strand, within BPHYT_RS36225 at 59.393 kb on - strand, within BPHYT_RS36225 at 59.393 kb on - strand, within BPHYT_RS36225 at 59.678 kb on + strand, within BPHYT_RS36230 at 59.678 kb on + strand, within BPHYT_RS36230 at 59.850 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1 remove 58,566 - +0.4 58,586 - BPHYT_RS36215 0.13 -0.1 58,774 - BPHYT_RS36215 0.49 +0.3 59,198 + BPHYT_RS36220 0.55 -3.3 59,329 + BPHYT_RS36225 0.23 -0.9 59,330 - BPHYT_RS36225 0.23 +0.4 59,362 - BPHYT_RS36225 0.35 -0.0 59,369 - BPHYT_RS36225 0.38 -0.8 59,392 + BPHYT_RS36225 0.46 +0.6 59,393 - BPHYT_RS36225 0.47 -0.8 59,393 - BPHYT_RS36225 0.47 -0.1 59,393 - BPHYT_RS36225 0.47 -0.5 59,393 - BPHYT_RS36225 0.47 -1.0 59,678 + BPHYT_RS36230 0.43 -0.6 59,678 + BPHYT_RS36230 0.43 -0.6 59,850 - -0.7
Or see this region's nucleotide sequence