Experiment: 36hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35445 and BPHYT_RS35450 are separated by 71 nucleotides BPHYT_RS35450 and BPHYT_RS35455 overlap by 4 nucleotides BPHYT_RS35455 and BPHYT_RS35460 overlap by 4 nucleotides
BPHYT_RS35445: BPHYT_RS35445 - 2-aminobenzoate-CoA ligase [EC: 6.2.1.32] <small>(from data)</small>, at 3,553,581 to 3,555,221
_RS35445
BPHYT_RS35450: BPHYT_RS35450 - endoribonuclease L-PSP, at 3,555,293 to 3,555,688
_RS35450
BPHYT_RS35455: BPHYT_RS35455 - 4-hydroxybenzoyl-CoA thioesterase, at 3,555,685 to 3,556,128
_RS35455
BPHYT_RS35460: BPHYT_RS35460 - acyl-CoA dehydrogenase, at 3,556,125 to 3,557,333
_RS35460
Position (kb) 3555
3556
3557 Strain fitness (log2 ratio) -2
-1
0
1 at 3554.903 kb on + strand, within BPHYT_RS35445 at 3555.459 kb on + strand, within BPHYT_RS35450 at 3555.886 kb on - strand, within BPHYT_RS35455 at 3556.403 kb on - strand, within BPHYT_RS35460 at 3556.517 kb on + strand, within BPHYT_RS35460 at 3556.852 kb on + strand, within BPHYT_RS35460 at 3556.852 kb on + strand, within BPHYT_RS35460 at 3556.853 kb on - strand, within BPHYT_RS35460
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1 remove 3,554,903 + BPHYT_RS35445 0.81 -2.1 3,555,459 + BPHYT_RS35450 0.42 +0.1 3,555,886 - BPHYT_RS35455 0.45 +1.4 3,556,403 - BPHYT_RS35460 0.23 +0.3 3,556,517 + BPHYT_RS35460 0.32 -0.1 3,556,852 + BPHYT_RS35460 0.60 -0.3 3,556,852 + BPHYT_RS35460 0.60 +0.3 3,556,853 - BPHYT_RS35460 0.60 -0.2
Or see this region's nucleotide sequence