Experiment: 36hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31220 and BPHYT_RS31225 are separated by 417 nucleotides BPHYT_RS31225 and BPHYT_RS31230 are separated by 64 nucleotides
BPHYT_RS31220: BPHYT_RS31220 - porin, at 2,562,979 to 2,564,088
_RS31220
BPHYT_RS31225: BPHYT_RS31225 - D-alanyl-D-alanine dipeptidase, at 2,564,506 to 2,565,069
_RS31225
BPHYT_RS31230: BPHYT_RS31230 - ABC transporter substrate-binding protein, at 2,565,134 to 2,566,741
_RS31230
Position (kb)
2564
2565
2566 Strain fitness (log2 ratio)
-1
0
1
2 at 2563.514 kb on - strand, within BPHYT_RS31220 at 2563.667 kb on - strand, within BPHYT_RS31220 at 2563.724 kb on - strand, within BPHYT_RS31220 at 2563.757 kb on - strand, within BPHYT_RS31220 at 2563.757 kb on - strand, within BPHYT_RS31220 at 2563.874 kb on - strand, within BPHYT_RS31220 at 2563.875 kb on + strand, within BPHYT_RS31220 at 2563.969 kb on + strand, within BPHYT_RS31220 at 2563.970 kb on - strand, within BPHYT_RS31220 at 2564.038 kb on - strand at 2564.145 kb on + strand at 2564.146 kb on - strand at 2564.237 kb on + strand at 2564.238 kb on - strand at 2564.399 kb on + strand at 2564.400 kb on - strand at 2564.400 kb on - strand at 2564.400 kb on - strand at 2564.401 kb on + strand at 2564.453 kb on + strand at 2564.606 kb on - strand, within BPHYT_RS31225 at 2564.606 kb on - strand, within BPHYT_RS31225 at 2564.711 kb on - strand, within BPHYT_RS31225 at 2564.764 kb on + strand, within BPHYT_RS31225 at 2564.940 kb on - strand, within BPHYT_RS31225 at 2565.123 kb on - strand at 2565.317 kb on + strand, within BPHYT_RS31230 at 2565.328 kb on + strand, within BPHYT_RS31230 at 2565.328 kb on + strand, within BPHYT_RS31230 at 2565.632 kb on + strand, within BPHYT_RS31230 at 2565.770 kb on + strand, within BPHYT_RS31230 at 2566.011 kb on - strand, within BPHYT_RS31230 at 2566.011 kb on - strand, within BPHYT_RS31230
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1 remove 2,563,514 - BPHYT_RS31220 0.48 +0.1 2,563,667 - BPHYT_RS31220 0.62 +0.6 2,563,724 - BPHYT_RS31220 0.67 -1.1 2,563,757 - BPHYT_RS31220 0.70 +0.3 2,563,757 - BPHYT_RS31220 0.70 +0.2 2,563,874 - BPHYT_RS31220 0.81 +0.6 2,563,875 + BPHYT_RS31220 0.81 +2.3 2,563,969 + BPHYT_RS31220 0.89 -1.5 2,563,970 - BPHYT_RS31220 0.89 -0.8 2,564,038 - +0.1 2,564,145 + -1.5 2,564,146 - +0.5 2,564,237 + +2.3 2,564,238 - -0.5 2,564,399 + -0.1 2,564,400 - +0.5 2,564,400 - +0.9 2,564,400 - +0.0 2,564,401 + +1.1 2,564,453 + +0.0 2,564,606 - BPHYT_RS31225 0.18 +0.8 2,564,606 - BPHYT_RS31225 0.18 -1.4 2,564,711 - BPHYT_RS31225 0.36 -0.2 2,564,764 + BPHYT_RS31225 0.46 -0.5 2,564,940 - BPHYT_RS31225 0.77 +2.5 2,565,123 - -0.4 2,565,317 + BPHYT_RS31230 0.11 -0.8 2,565,328 + BPHYT_RS31230 0.12 +0.9 2,565,328 + BPHYT_RS31230 0.12 +0.0 2,565,632 + BPHYT_RS31230 0.31 +0.5 2,565,770 + BPHYT_RS31230 0.40 +0.7 2,566,011 - BPHYT_RS31230 0.55 +0.0 2,566,011 - BPHYT_RS31230 0.55 -0.4
Or see this region's nucleotide sequence