Experiment: 36hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30830 and BPHYT_RS30835 are separated by 82 nucleotides BPHYT_RS30835 and BPHYT_RS30840 are separated by 154 nucleotides BPHYT_RS30840 and BPHYT_RS30845 are separated by 227 nucleotides BPHYT_RS30845 and BPHYT_RS30850 are separated by 230 nucleotides
BPHYT_RS30830: BPHYT_RS30830 - MFS transporter, at 2,472,546 to 2,473,859
_RS30830
BPHYT_RS30835: BPHYT_RS30835 - hypothetical protein, at 2,473,942 to 2,474,304
_RS30835
BPHYT_RS30840: BPHYT_RS30840 - small mechanosensitive ion channel protein MscS, at 2,474,459 to 2,475,673
_RS30840
BPHYT_RS30845: BPHYT_RS30845 - polyketide cyclase, at 2,475,901 to 2,476,389
_RS30845
BPHYT_RS30850: BPHYT_RS30850 - MarR family transcriptional regulator, at 2,476,620 to 2,477,054
_RS30850
Position (kb)
2474
2475
2476 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2473.888 kb on + strand at 2473.889 kb on - strand at 2473.892 kb on + strand at 2473.943 kb on + strand at 2474.427 kb on + strand at 2474.481 kb on + strand at 2474.527 kb on - strand at 2474.555 kb on + strand at 2475.190 kb on - strand, within BPHYT_RS30840 at 2475.190 kb on - strand, within BPHYT_RS30840 at 2475.318 kb on + strand, within BPHYT_RS30840 at 2475.591 kb on - strand at 2475.661 kb on + strand at 2475.738 kb on - strand at 2475.857 kb on + strand at 2476.041 kb on + strand, within BPHYT_RS30845 at 2476.216 kb on - strand, within BPHYT_RS30845 at 2476.404 kb on + strand at 2476.405 kb on - strand at 2476.537 kb on - strand at 2476.541 kb on + strand at 2476.542 kb on - strand at 2476.638 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1 remove 2,473,888 + -0.7 2,473,889 - +0.9 2,473,892 + -0.0 2,473,943 + +0.1 2,474,427 + -1.6 2,474,481 + -2.3 2,474,527 - +1.4 2,474,555 + -2.9 2,475,190 - BPHYT_RS30840 0.60 +0.4 2,475,190 - BPHYT_RS30840 0.60 -0.9 2,475,318 + BPHYT_RS30840 0.71 -0.1 2,475,591 - -0.2 2,475,661 + -0.8 2,475,738 - -0.8 2,475,857 + +0.0 2,476,041 + BPHYT_RS30845 0.29 -1.1 2,476,216 - BPHYT_RS30845 0.64 +0.4 2,476,404 + -0.3 2,476,405 - -0.9 2,476,537 - +0.8 2,476,541 + -1.3 2,476,542 - -0.1 2,476,638 + -1.9
Or see this region's nucleotide sequence