Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28625

Experiment: 36hr PHYTO6 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28620 and BPHYT_RS28625 are separated by 285 nucleotidesBPHYT_RS28625 and BPHYT_RS28630 are separated by 13 nucleotidesBPHYT_RS28630 and BPHYT_RS28635 are separated by 87 nucleotides BPHYT_RS28620: BPHYT_RS28620 - enoyl-CoA hydratase, at 1,975,406 to 1,976,188 _RS28620 BPHYT_RS28625: BPHYT_RS28625 - major facilitator transporter, at 1,976,474 to 1,977,805 _RS28625 BPHYT_RS28630: BPHYT_RS28630 - IclR family transcriptional regulator, at 1,977,819 to 1,978,610 _RS28630 BPHYT_RS28635: BPHYT_RS28635 - hypothetical protein, at 1,978,698 to 1,978,919 _RS28635 Position (kb) 1976 1977 1978Strain fitness (log2 ratio) -1 0 1at 1975.731 kb on + strand, within BPHYT_RS28620at 1975.770 kb on + strand, within BPHYT_RS28620at 1975.771 kb on - strand, within BPHYT_RS28620at 1975.771 kb on - strand, within BPHYT_RS28620at 1976.195 kb on - strandat 1976.307 kb on + strandat 1976.405 kb on + strandat 1976.837 kb on + strand, within BPHYT_RS28625at 1977.635 kb on - strand, within BPHYT_RS28625at 1977.725 kb on + strandat 1978.388 kb on - strand, within BPHYT_RS28630at 1978.417 kb on + strand, within BPHYT_RS28630at 1978.589 kb on + strandat 1978.699 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1
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1,975,731 + BPHYT_RS28620 0.42 +0.8
1,975,770 + BPHYT_RS28620 0.46 +1.2
1,975,771 - BPHYT_RS28620 0.47 +0.1
1,975,771 - BPHYT_RS28620 0.47 +0.8
1,976,195 - -0.3
1,976,307 + -1.5
1,976,405 + +0.2
1,976,837 + BPHYT_RS28625 0.27 -0.7
1,977,635 - BPHYT_RS28625 0.87 -1.3
1,977,725 + +0.2
1,978,388 - BPHYT_RS28630 0.72 +0.8
1,978,417 + BPHYT_RS28630 0.76 -0.2
1,978,589 + -0.0
1,978,699 + +1.7

Or see this region's nucleotide sequence