Experiment: 36hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26895 and BPHYT_RS26900 are separated by 82 nucleotides BPHYT_RS26900 and BPHYT_RS26905 are separated by 214 nucleotides BPHYT_RS26905 and BPHYT_RS26910 are separated by 67 nucleotides
BPHYT_RS26895: BPHYT_RS26895 - nicotinate phosphoribosyltransferase, at 1,606,296 to 1,607,723
_RS26895
BPHYT_RS26900: BPHYT_RS26900 - isoprenylcysteine carboxyl methyltransferase, at 1,607,806 to 1,608,492
_RS26900
BPHYT_RS26905: BPHYT_RS26905 - pilus assembly protein CpaE, at 1,608,707 to 1,609,909
_RS26905
BPHYT_RS26910: BPHYT_RS26910 - hypothetical protein, at 1,609,977 to 1,610,264
_RS26910
Position (kb) 1608
1609
1610 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1607.931 kb on - strand, within BPHYT_RS26900 at 1608.350 kb on + strand, within BPHYT_RS26900 at 1608.370 kb on - strand, within BPHYT_RS26900 at 1610.232 kb on + strand, within BPHYT_RS26910 at 1610.232 kb on + strand, within BPHYT_RS26910 at 1610.294 kb on - strand at 1610.696 kb on + strand at 1610.783 kb on - strand at 1610.783 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1 remove 1,607,931 - BPHYT_RS26900 0.18 -2.8 1,608,350 + BPHYT_RS26900 0.79 -0.7 1,608,370 - BPHYT_RS26900 0.82 -0.5 1,610,232 + BPHYT_RS26910 0.89 +1.2 1,610,232 + BPHYT_RS26910 0.89 +0.4 1,610,294 - -1.2 1,610,696 + +0.7 1,610,783 - -0.7 1,610,783 - +1.1
Or see this region's nucleotide sequence