Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25875

Experiment: 36hr PHYTO6 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25870 and BPHYT_RS25875 are separated by 12 nucleotidesBPHYT_RS25875 and BPHYT_RS25880 are separated by 1 nucleotidesBPHYT_RS25880 and BPHYT_RS25885 are separated by 32 nucleotides BPHYT_RS25870: BPHYT_RS25870 - type III secretion system ATPase, at 1,383,596 to 1,384,924 _RS25870 BPHYT_RS25875: BPHYT_RS25875 - type III secretion protein, at 1,384,937 to 1,387,012 _RS25875 BPHYT_RS25880: BPHYT_RS25880 - type III secretion protein, at 1,387,014 to 1,387,754 _RS25880 BPHYT_RS25885: BPHYT_RS25885 - type III secretion protein, at 1,387,787 to 1,388,476 _RS25885 Position (kb) 1384 1385 1386 1387 1388Strain fitness (log2 ratio) -2 -1 0 1at 1384.676 kb on - strand, within BPHYT_RS25870at 1384.910 kb on + strandat 1386.304 kb on - strand, within BPHYT_RS25875at 1386.445 kb on - strand, within BPHYT_RS25875at 1386.885 kb on - strandat 1387.760 kb on + strandat 1387.760 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1
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1,384,676 - BPHYT_RS25870 0.81 -1.9
1,384,910 + -0.6
1,386,304 - BPHYT_RS25875 0.66 -1.1
1,386,445 - BPHYT_RS25875 0.73 -0.7
1,386,885 - -1.5
1,387,760 + -0.1
1,387,760 + +0.4

Or see this region's nucleotide sequence