Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25660

Experiment: 36hr PHYTO6 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25650 and BPHYT_RS25655 are separated by 111 nucleotidesBPHYT_RS25655 and BPHYT_RS25660 are separated by 2 nucleotidesBPHYT_RS25660 and BPHYT_RS25665 are separated by 204 nucleotides BPHYT_RS25650: BPHYT_RS25650 - dihydroxy-acid dehydratase, at 1,327,200 to 1,328,966 _RS25650 BPHYT_RS25655: BPHYT_RS25655 - short-chain dehydrogenase, at 1,329,078 to 1,329,878 _RS25655 BPHYT_RS25660: BPHYT_RS25660 - MFS transporter, at 1,329,881 to 1,331,239 _RS25660 BPHYT_RS25665: BPHYT_RS25665 - LysR family transcriptional regulator, at 1,331,444 to 1,332,418 _RS25665 Position (kb) 1329 1330 1331 1332Strain fitness (log2 ratio) -1 0 1at 1329.505 kb on + strand, within BPHYT_RS25655at 1330.435 kb on - strand, within BPHYT_RS25660at 1330.689 kb on + strand, within BPHYT_RS25660at 1330.879 kb on - strand, within BPHYT_RS25660at 1330.879 kb on - strand, within BPHYT_RS25660at 1331.011 kb on - strand, within BPHYT_RS25660at 1332.189 kb on + strand, within BPHYT_RS25665

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1
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1,329,505 + BPHYT_RS25655 0.53 -0.8
1,330,435 - BPHYT_RS25660 0.41 +0.0
1,330,689 + BPHYT_RS25660 0.59 +0.1
1,330,879 - BPHYT_RS25660 0.73 -0.3
1,330,879 - BPHYT_RS25660 0.73 +0.2
1,331,011 - BPHYT_RS25660 0.83 +1.0
1,332,189 + BPHYT_RS25665 0.76 -0.1

Or see this region's nucleotide sequence