Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25650

Experiment: 36hr PHYTO6 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25640 and BPHYT_RS25645 are separated by 91 nucleotidesBPHYT_RS25645 and BPHYT_RS25650 overlap by 4 nucleotidesBPHYT_RS25650 and BPHYT_RS25655 are separated by 111 nucleotidesBPHYT_RS25655 and BPHYT_RS25660 are separated by 2 nucleotides BPHYT_RS25640: BPHYT_RS25640 - MFS transporter, at 1,324,884 to 1,326,218 _RS25640 BPHYT_RS25645: BPHYT_RS25645 - dehydrogenase, at 1,326,310 to 1,327,203 _RS25645 BPHYT_RS25650: BPHYT_RS25650 - dihydroxy-acid dehydratase, at 1,327,200 to 1,328,966 _RS25650 BPHYT_RS25655: BPHYT_RS25655 - short-chain dehydrogenase, at 1,329,078 to 1,329,878 _RS25655 BPHYT_RS25660: BPHYT_RS25660 - MFS transporter, at 1,329,881 to 1,331,239 _RS25660 Position (kb) 1327 1328 1329Strain fitness (log2 ratio) -2 -1 0 1at 1326.244 kb on + strandat 1326.245 kb on - strandat 1326.246 kb on + strandat 1326.433 kb on - strand, within BPHYT_RS25645at 1327.003 kb on + strand, within BPHYT_RS25645at 1327.422 kb on - strand, within BPHYT_RS25650at 1328.205 kb on - strand, within BPHYT_RS25650at 1328.621 kb on - strand, within BPHYT_RS25650at 1328.625 kb on - strand, within BPHYT_RS25650at 1329.505 kb on + strand, within BPHYT_RS25655

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO6 MOI 1
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1,326,244 + -1.2
1,326,245 - -0.1
1,326,246 + +0.2
1,326,433 - BPHYT_RS25645 0.14 -2.7
1,327,003 + BPHYT_RS25645 0.78 -1.3
1,327,422 - BPHYT_RS25650 0.13 -0.8
1,328,205 - BPHYT_RS25650 0.57 -1.0
1,328,621 - BPHYT_RS25650 0.80 -0.6
1,328,625 - BPHYT_RS25650 0.81 -0.6
1,329,505 + BPHYT_RS25655 0.53 -0.8

Or see this region's nucleotide sequence